{"title":"Characterization and evaluation of Bacillus altitudinis BS-4 as a novel potential biocontrol agent against Phytophthora sojae in soybean","authors":"Shun Cao, Fangxin Chen, Yuli Dai, Zhenyu Zhao, Bingxin Jiang, Yuemin Pan, Zhimou Gao","doi":"10.1007/s40858-024-00637-5","DOIUrl":null,"url":null,"abstract":"<p>Soybean Phytophthora root rot (PRR) caused by <i>Phytophthora sojae</i> is one of the most devastating diseases of soybean worldwide. This study was conducted to develop new effective biocontrol agents for the control of the disease and explore the action mechanism<i>.</i> Eleven antagonistic bacterial strains against <i>P. sojae</i> were isolated from the soybean field soil samples, and among the strains, BS-4 not only had the strongest inhibitory activity against <i>P. sojae</i>, but also had good inhibition to other four species of oomycetes in <i>Phytophthora</i> and eight fungal pathogens. Based on 16S rDNA sequence analysis and the physiological and biochemical properties, the strain BS-4 was identified as <i>Bacillus altitudinis</i>. Strain BS-4 culture filtrate had significant inhibitory effects on the pathogens, and the inhibition rates were stronger than those in the dual culture. BS-4 filtrate kept a high bioactivity to <i>P. sojae</i> at a high temperature, even at 121℃, and in the pH range of 5 to 12. The germination of zoospores and the formation of sexual organs were seriously affected by BS-4 filtrate. Furthermore, detached leaf assays and greenhouse experiments showed that BS-4 suspension and culture filtrate all had good disease control efficacy, and in the field experiment, BS-4 suspension reduced the disease index and increased the biomass of soybean shoots and roots, significantly (<i>P</i> < 0.05), with control effect of 75.29% on PRR. It is suggested that <i>B. altitudinis</i> strain BS-4 is a novel potential biocontrol agent to control PRR, and will have a broad application prospect in PRR biocontrol.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00637-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Soybean Phytophthora root rot (PRR) caused by Phytophthora sojae is one of the most devastating diseases of soybean worldwide. This study was conducted to develop new effective biocontrol agents for the control of the disease and explore the action mechanism. Eleven antagonistic bacterial strains against P. sojae were isolated from the soybean field soil samples, and among the strains, BS-4 not only had the strongest inhibitory activity against P. sojae, but also had good inhibition to other four species of oomycetes in Phytophthora and eight fungal pathogens. Based on 16S rDNA sequence analysis and the physiological and biochemical properties, the strain BS-4 was identified as Bacillus altitudinis. Strain BS-4 culture filtrate had significant inhibitory effects on the pathogens, and the inhibition rates were stronger than those in the dual culture. BS-4 filtrate kept a high bioactivity to P. sojae at a high temperature, even at 121℃, and in the pH range of 5 to 12. The germination of zoospores and the formation of sexual organs were seriously affected by BS-4 filtrate. Furthermore, detached leaf assays and greenhouse experiments showed that BS-4 suspension and culture filtrate all had good disease control efficacy, and in the field experiment, BS-4 suspension reduced the disease index and increased the biomass of soybean shoots and roots, significantly (P < 0.05), with control effect of 75.29% on PRR. It is suggested that B. altitudinis strain BS-4 is a novel potential biocontrol agent to control PRR, and will have a broad application prospect in PRR biocontrol.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.