Characterization and evaluation of Bacillus altitudinis BS-4 as a novel potential biocontrol agent against Phytophthora sojae in soybean

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Tropical Plant Pathology Pub Date : 2024-02-26 DOI:10.1007/s40858-024-00637-5
Shun Cao, Fangxin Chen, Yuli Dai, Zhenyu Zhao, Bingxin Jiang, Yuemin Pan, Zhimou Gao
{"title":"Characterization and evaluation of Bacillus altitudinis BS-4 as a novel potential biocontrol agent against Phytophthora sojae in soybean","authors":"Shun Cao, Fangxin Chen, Yuli Dai, Zhenyu Zhao, Bingxin Jiang, Yuemin Pan, Zhimou Gao","doi":"10.1007/s40858-024-00637-5","DOIUrl":null,"url":null,"abstract":"<p>Soybean Phytophthora root rot (PRR) caused by <i>Phytophthora sojae</i> is one of the most devastating diseases of soybean worldwide. This study was conducted to develop new effective biocontrol agents for the control of the disease and explore the action mechanism<i>.</i> Eleven antagonistic bacterial strains against <i>P. sojae</i> were isolated from the soybean field soil samples, and among the strains, BS-4 not only had the strongest inhibitory activity against <i>P. sojae</i>, but also had good inhibition to other four species of oomycetes in <i>Phytophthora</i> and eight fungal pathogens. Based on 16S rDNA sequence analysis and the physiological and biochemical properties, the strain BS-4 was identified as <i>Bacillus altitudinis</i>. Strain BS-4 culture filtrate had significant inhibitory effects on the pathogens, and the inhibition rates were stronger than those in the dual culture. BS-4 filtrate kept a high bioactivity to <i>P. sojae</i> at a high temperature, even at 121℃, and in the pH range of 5 to 12. The germination of zoospores and the formation of sexual organs were seriously affected by BS-4 filtrate. Furthermore, detached leaf assays and greenhouse experiments showed that BS-4 suspension and culture filtrate all had good disease control efficacy, and in the field experiment, BS-4 suspension reduced the disease index and increased the biomass of soybean shoots and roots, significantly (<i>P</i> &lt; 0.05), with control effect of 75.29% on PRR. It is suggested that <i>B. altitudinis</i> strain BS-4 is a novel potential biocontrol agent to control PRR, and will have a broad application prospect in PRR biocontrol.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":"66 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00637-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Soybean Phytophthora root rot (PRR) caused by Phytophthora sojae is one of the most devastating diseases of soybean worldwide. This study was conducted to develop new effective biocontrol agents for the control of the disease and explore the action mechanism. Eleven antagonistic bacterial strains against P. sojae were isolated from the soybean field soil samples, and among the strains, BS-4 not only had the strongest inhibitory activity against P. sojae, but also had good inhibition to other four species of oomycetes in Phytophthora and eight fungal pathogens. Based on 16S rDNA sequence analysis and the physiological and biochemical properties, the strain BS-4 was identified as Bacillus altitudinis. Strain BS-4 culture filtrate had significant inhibitory effects on the pathogens, and the inhibition rates were stronger than those in the dual culture. BS-4 filtrate kept a high bioactivity to P. sojae at a high temperature, even at 121℃, and in the pH range of 5 to 12. The germination of zoospores and the formation of sexual organs were seriously affected by BS-4 filtrate. Furthermore, detached leaf assays and greenhouse experiments showed that BS-4 suspension and culture filtrate all had good disease control efficacy, and in the field experiment, BS-4 suspension reduced the disease index and increased the biomass of soybean shoots and roots, significantly (P < 0.05), with control effect of 75.29% on PRR. It is suggested that B. altitudinis strain BS-4 is a novel potential biocontrol agent to control PRR, and will have a broad application prospect in PRR biocontrol.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
表征和评估高度芽孢杆菌 BS-4 作为一种新型潜在生物控制剂对大豆根腐病的防治效果
Phytophthora sojae 引起的大豆疫霉根腐病(PRR)是全球最具毁灭性的大豆病害之一。本研究旨在开发新的有效生物防治剂来控制该病害,并探索其作用机制。研究人员从大豆田土壤样品中分离出 11 株拮抗大豆疫霉的细菌菌株,其中 BS-4 菌株不仅对大豆疫霉具有最强的抑制活性,而且对疫霉中的其他 4 种卵菌和 8 种真菌病原菌也有很好的抑制作用。根据 16S rDNA 序列分析和生理生化特性,确定菌株 BS-4 为高纬度芽孢杆菌。菌株 BS-4 的培养滤液对病原菌有明显的抑制作用,抑制率强于双培养物。BS-4 菌株的滤液在高温(甚至 121℃)和 pH 值为 5 至 12 的范围内对 P. sojae 保持较高的生物活性。BS-4 滤液严重影响了动物孢子的萌发和性器官的形成。此外,离体叶片测定和温室实验表明,BS-4悬浮剂和培养滤液都具有良好的病害防治效果,在田间试验中,BS-4悬浮剂显著降低了病害指数,增加了大豆芽和根的生物量(P <0.05),对PRR的防治效果达75.29%。这表明,B. altitudinis 菌株 BS-4 是一种新型的潜在生物防治 PRR 的菌剂,在 PRR 生物防治方面具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
期刊最新文献
Genetic variability and population structure of Pseudocercospora griseola isolates in the Black Sea region of Türkiye Identification of the causal agent of Guar leaf blight and development of a semi-automated method to quantify disease severity Genomic analysis identifies five pathogenic bacterial species in Argentinian wheat Detached leaf assay-based screening of anthracnose resistant wild strawberry genotypes Spatiotemporal dynamics of Fusarium wilt of banana caused by Subtropical Race 4
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1