Qiming Chen, Yang Yu, Xiaodi Chen, Fangming Tu, Peng Wang, Junyi Huang, Zhanmin Liu
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引用次数: 0
Abstract
Cronobacter sakazakii in powdered infant formula (PIF) can infect infants with a mortality rate of up to 80%. In this study, a propidium monoazide–loop-mediated isothermal amplification (PMA-LAMP) assay was developed for fast and cost-effective visual detection of viable C. sakazakii. The detection could be finished in 75 min and the results could be identified by the naked eye. The detection limits of the assay were as low as 1.2 × 101 cfu/mL and 1.2 × 102 cfu/mL for gel electrophoresis and naked eyes respectively. Finally, the assay also showed good reliability in the artificially contaminated PIF.
期刊介绍：
The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope.
Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.
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