Extraction Optimization, Structure Analysis and Antioxidant Activity of Polysaccharide from Sanghuangporus baumii

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-28 DOI:10.2174/0115734110289728240214103704
Yunhe Qu, Pingping Zhang, Jing Cui, Xiuzhen Ni, Kai Song, Dongfang Shi
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Abstract

Introduction:: In this study, we optimized the extraction process, analyzed the structure and assessed the antioxidant activity of Sanghuangporus baumii polysaccharide (SBP). The present results provide important information for the Sanghuangporus baumii polysaccharides in potential natural antioxidant effects. Background:: The extraction and structural analysis of polysaccharides from Sanghuangporus has gained significant attention in the fields of chemistry, medicine, and life sciences. There is great significance in maximizing the extraction of polysaccharides from Sanghuangporus and developing their potential products in a scientific and rational manner. Objective:: The study was designed to establish an efficient and practical extraction process for SBP, and then investigated the structure and the antioxidant activity. Methods:: The Response Surface Methodology (RSM) based on Box-Behnken design was used to explore the ultrasound-assisted extraction of SBP, and the structure of SBP was studied by ultraviolet spectroscopy, infrared spectroscopy and other instrumental analysis methods. The total antioxidant capacity of SBP was studied by the Ferric Reducing Ability of Plasma (FRAP) method, and the scavenging capacity of ABTS+·, DPPH· and OH· was carried out as the index to investigate its antioxidant activity in vitro. Results:: The statistical analysis results showed that the optimal conditions for extracting SBP were an ultrasound time of 20.74 min, ultrasound power of 268.40 W and material-liquid ratio of 1:25.71. Under optimal conditions, the experimental yield of SBP was 3.36 ± 0.01%. The RSM optimization process was applied to the experiment of complex enzyme extraction of SBP, and the yield increased to 4.72 ± 0.03%. Structural analysis showed that SBP mainly consisted of glucose, a small amount of mannose and galactose, and the molecular weight distribution was uneven, mainly concentrating in the three parts of 24.5,6.4,2.5 kDa. Moreover, SBP exhibited dose-dependent and strong reducing power and radical scavenging activity. For DPPH·, ABTS+· and OH· radical scavenging assays, IC50 values were 1.505 ± 0.050 mg/ml, 0.065 ± 0.036 mg/ml and 0.442 ± 0.007 mg/ml, respectively. Conclusion:: In the present study, a β-linked heteroglucan (SBP) was extracted using the optimized process combining enzymes from the fruiting bodies of Sanghuangporus baumii. SBP exhibited effective and dose-dependent antioxidant activities. Our findings were of great value in terms of developing polysaccharides with potential natural antioxidants.
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桑黄柏多糖的提取优化、结构分析和抗氧化活性
引言本研究优化了桑黄多糖的提取工艺,分析了其结构,并评估了其抗氧化活性。本研究结果为黄桑蚕多糖潜在的天然抗氧化作用提供了重要信息。研究背景从桑黄菌中提取多糖并对其进行结构分析已在化学、医学和生命科学领域受到广泛关注。最大限度地提取桑黄多糖,科学合理地开发其潜在产品具有重要意义。研究目的本研究旨在建立一种高效实用的 SBP 提取工艺,并对其结构和抗氧化活性进行研究。方法采用基于方框-贝肯设计的响应面方法(RSM)对超声辅助提取枸杞多糖进行了探讨,并通过紫外光谱、红外光谱等仪器分析方法对枸杞多糖的结构进行了研究。采用血浆铁还原能力(FRAP)法研究了SBP的总抗氧化能力,并以ABTS+-、DPPH-和OH-的清除能力为指标研究了其体外抗氧化活性。结果表明统计分析结果表明,提取 SBP 的最佳条件是超声时间为 20.74 分钟,超声功率为 268.40 W,料液比为 1:25.71。在最佳条件下,SBP 的实验收率为 3.36 ± 0.01%。将 RSM 优化过程应用于复合酶提取 SBP 的实验,收率提高到 4.72 ± 0.03%。结构分析表明,SBP 主要由葡萄糖、少量甘露糖和半乳糖组成,分子量分布不均,主要集中在 24.5、6.4、2.5 kDa 三部分。此外,SBP 还具有剂量依赖性和较强的还原力和自由基清除活性。在 DPPH-、ABTS+- 和 OH- 自由基清除实验中,IC50 值分别为 1.505 ± 0.050 mg/ml、0.065 ± 0.036 mg/ml 和 0.442 ± 0.007 mg/ml。结论在本研究中,采用优化的工艺结合酶从鲍曼不动杆菌子实体中提取出了β-连接异葡聚糖(SBP)。SBP 表现出有效的、剂量依赖性的抗氧化活性。我们的研究结果对开发具有潜在天然抗氧化剂的多糖具有重要价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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