Application of the plankton-kreisel tank for small-scale larviculture of Pacific bluefin tuna Thunnus orientalis

IF 1.4 4区农林科学 Q3 FISHERIES Fisheries Science Pub Date : 2024-02-29 DOI:10.1007/s12562-024-01762-5

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Abstract

Genome editing by manipulating the embryos of Pacific bluefin tuna (PBT) was recently proposed for improving the breeding and aquaculture production of PBT. However, the yield of genome-edited eggs is limited due to the narrow timing of genome editing of embryos and the labor-intensive process. Therefore, the development of a small-scale larviculture method is necessary for efficient evaluation of the phenotype and traits of genome-edited PBT larvae. The plankton-kreisel tank can form a vertical rotating flow that may prevent the sinking syndrome of PBT larvae. In this study, we applied a plankton-kreisel tank (8-L) for PBT larviculture up to 10 days post-hatch (dph). We compared the survival rate and growth of PBT larvae reared in the 8-L plankton-kreisel tank and an 8-L cylindrical tank (CT). The survival rate in the plankton-kreisel tank at 10 dph (58.9 ± 4.8%) was significantly higher than that in the CT (4.8 ± 3.6%). Larval growth was not significantly different between these tanks. We observed that the larvae that sank to the tank bottom drifted with the strong vertical rotating flow along the tank wall during the night. This flow in the plankton-kreisel tank prevents the sinking syndrome. Thus, this apparatus is proposed for small-scale larviculture experiments in PBT.

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在小规模养殖太平洋蓝鳍金枪鱼（东方鲣）过程中应用浮游生物-克雷塞尔池

摘要最近有人提出通过操作太平洋蓝鳍金枪鱼（PBT）胚胎进行基因组编辑，以改进太平洋蓝鳍金枪鱼的育种和水产养殖生产。然而，由于胚胎基因组编辑的时间较窄，且过程耗费人力，基因组编辑卵的产量有限。因此，有必要开发一种小规模幼体培养方法，以有效评估基因组编辑 PBT 幼体的表型和性状。浮游生物饲养箱可形成垂直旋转流，可防止 PBT 幼虫的下沉综合症。在本研究中，我们将浮游生物-克里舍尔水槽（8 升）用于孵化后 10 天（dph）内的 PBT 幼虫养殖。我们比较了在 8 升浮游生物饲养箱和 8 升圆柱形饲养箱（CT）中饲养的 PBT 幼虫的存活率和生长情况。在 10 dph 时，浮游生物-克里赛尔水槽中的存活率（58.9 ± 4.8%）明显高于 CT 中的存活率（4.8 ± 3.6%）。幼虫的生长在这些水槽中没有明显差异。我们观察到，沉入水槽底部的幼虫在夜间会随着水槽壁上强大的垂直旋转流漂移。浮游生物-克雷塞尔水槽中的这种流动防止了沉底综合症。因此，建议将这种装置用于 PBT 的小规模幼虫养殖实验。

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来源期刊

Fisheries Science 农林科学-渔业

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

12-24 weeks

期刊介绍： Fisheries Science is the official journal of the Japanese Society of Fisheries Science, which was established in 1932. Recognized as a leading journal in its field, Fisheries Science is respected internationally for the publication of basic and applied research articles in a broad range of subject areas relevant to fisheries science. All articles are peer-reviewed by at least two experts in the field of the submitted paper. Published six times per year, Fisheries Science includes about 120 articles per volume. It has a rich history of publishing quality papers in fisheries, biology, aquaculture, environment, chemistry and biochemistry, food science and technology, and Social Science.