Comparison of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY Practical Laboratory Medicine Pub Date : 2024-03-01 DOI:10.1016/j.plabm.2024.e00386

Sedat Abusoglu , Halil Guven , Busra Ecer , Ahmet Emre Yorulmaz , Abdullah Sivrikaya , Fatma Humeyra Yerlikaya Aydemir , Ali Unlu , Gulsum Abusoglu , Muhittin Abdulkadir Serdar

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Abstract

Objectives

Urinalysis is a first-line test for screening for urinary tract infection. Several devices performing strip and sediment analysis have been introduced. The aim of this study was to compare the performance of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy.

Methods

463 urine samples were analyzed. Digital image processing and particle recognition automatically display the cells in a flowing sheath fluid mixed monolayer urine sample, take the pictures of particles via digital camera, analyse these pics with a particle recognition software, transfer images of the formed elements to the screen and allow well-trained personnel to select, reclassify or remove them. Manual microscopy was used for comparison.

Results

Agreement between Tricell-100 and manual microscopy was very good for RBC (ϰ = 0.80), and WBC (ϰ = 0.83); good for CaOx (ϰ = 0.69), SEC (ϰ = 0.80), YLC (ϰ = 0.72), HC (0.69) and LC (ϰ = 0.64); moderate for BAC (ϰ = 0.51), APC (ϰ = 0.43) and MT (ϰ = 0.55); fair for GC (ϰ = 0.39) and RTEC (ϰ = 0.32).

Conclusions

Labsan Trion TriCell-1000 demonstrated satisfactory performance and can be used in routine urinalysis. In the case of low counts of RBC, presence of yeast, crystal, casts or cell clumping in urine sediment, characterization of urine particles should be performed by manual microscopy.

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Labsan Tricell-1000 和 Dirui FUS-2000 自动尿液分析仪与人工显微镜的比较

目的尿液分析是筛查尿路感染的一线检测方法。目前已推出了几种可进行条带和沉淀物分析的设备。本研究旨在比较 Labsan Tricell-1000 和 Dirui FUS-2000 自动尿液分析仪与人工显微镜的性能。数字图像处理和颗粒识别自动显示流动的鞘液混合单层尿样中的细胞，通过数码相机拍摄颗粒照片，用颗粒识别软件分析这些照片，将形成的元素图像传输到屏幕上，并允许训练有素的人员选择、重新分类或删除它们。结果对于 RBC（ϰ = 0.80）和 WBC（ϰ = 0.83），Tricell-100 与手动显微镜的一致性非常好；对于 CaOx（ϰ = 0.69）、SEC（ϰ = 0.80）、YLC（ϰ = 0.72）、HC（0.69）和 LC（ϰ = 0.结论Labsan Trion TriCell-1000 的性能令人满意，可用于常规尿液分析。如果尿沉渣中的红细胞计数较低、存在酵母菌、晶体、铸型或细胞团块，则应通过人工显微镜对尿液颗粒进行定性。

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.