Evaluation of the Effects of Opium on the Expression of SOX2 and OCT4 in Wistar Rat Bladder

IF 1 4区医学 Q4 ONCOLOGY Bladder Cancer Pub Date : 2024-02-28 DOI:10.3233/blc-230076

Iman Menbari Oskouie, Leila Zareian Baghdadabad, Rahil Mashhadi, Parisa Zahmatkesh, Akram Mirzaei, Alireza Khajavi, Maryam Noori, Gholamreza Mesbah, Seyed Mohammad Kazem Aghamir

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Abstract

BACKGROUND:

Bladder cancer is a malignancy greatly affected by behavioral habits. The aim of this study was to examine the effect of opium on changes in the expression of OCT4 and SOX2 in the bladder tissue of rats.

METHOD:

Thirty six rats were divided into six groups: 24 rats in the addicted group received morphine and opium for 4 months with 12 rats in the control group. Blood testing was done for the evaluation of CBC, MDA, and TAC. The bladder tissue was removed and checked by histopathological examination. All total RNA was extracted, then cDNAs were synthesized and the OCT4 and SOX2 gene expressions were evaluated by Real-time PCR.

RESULTS:

The OCT4 mRNA expression level in the opium group of rats was significantly increased compared to the control group (13.5 and 6.8 fold in males and females respectively). Also, in the morphine group, similar augmentation was detected (3.8 and 6.7 fold in males and females respectively). The SOX2 mRNA over-expression level was seen in the morphine group of both genders as compared to the control group (3.7 and 4.2 fold in male and female respectively) but in the opium group, enhancement of mRNA level was seen only in males (6.6 fold). Opium increases both OCT4 and SOX2 expression more than morphine in male rats, but in female rats, SOX2 is increased more by morphine.

CONCLUSION:

Over expression of OCT4 and SOX2 was observed in rats treated with opium and morphine. Increased OCT4 and SOX2 expression was seen in opium-treated male rats, but in female rats, SOX2 was increased more by morphine.

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评估鸦片对 Wistar 大鼠膀胱中 SOX2 和 OCT4 表达的影响

背景：膀胱癌是一种受行为习惯影响较大的恶性肿瘤。方法：将 36 只大鼠分为 6 组：成瘾组 24 只大鼠接受吗啡和鸦片治疗 4 个月，对照组 12 只大鼠接受吗啡和鸦片治疗 4 个月。对大鼠进行血液检测，以评估 CBC、MDA 和 TAC。切除膀胱组织并进行组织病理学检查。结果：与对照组相比，鸦片组大鼠的 OCT4 mRNA 表达水平显著增加（雄性和雌性分别为 13.5 倍和 6.8 倍）。吗啡组大鼠的 OCT4 mRNA 表达水平也出现了类似的增加（雄性大鼠和雌性大鼠分别增加了 3.8 倍和 6.7 倍）。与对照组相比，吗啡组男女的 SOX2 mRNA 表达水平都有所提高（男性和女性分别为 3.7 倍和 4.2 倍），但在鸦片组中，只有男性的 mRNA 水平有所提高（6.6 倍）。在雄性大鼠中，鸦片比吗啡更能增加 OCT4 和 SOX2 的表达，但在雌性大鼠中，吗啡更能增加 SOX2 的表达。经鸦片处理的雄性大鼠的OCT4和SOX2表达增加，但在雌性大鼠中，吗啡对SOX2的影响更大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bladder Cancer Medicine-Urology

CiteScore

1.60

自引率

0.00%

发文量

期刊介绍： Bladder Cancer is an international multidisciplinary journal to facilitate progress in understanding the epidemiology/etiology, genetics, molecular correlates, pathogenesis, pharmacology, ethics, patient advocacy and survivorship, diagnosis and treatment of tumors of the bladder and upper urinary tract. The journal publishes research reports, reviews, short communications, and letters-to-the-editor. The journal is dedicated to providing an open forum for original research in basic science, translational research and clinical medicine that expedites our fundamental understanding and improves treatment of tumors of the bladder and upper urinary tract.