The Association of miRNA-146a Gene Variation and Multiple Sclerosis in The Iranian Population.

Q2 Medicine Medical Journal of the Islamic Republic of Iran Pub Date : 2024-01-08 eCollection Date: 2024-01-01 DOI:10.47176/mjiri.38.1

Atefeh Zamani, Masoumeh Dehghan Manshadi, Mansoureh Akouchekian, Iman Salahshouri Far

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Abstract

Background: Multiple sclerosis (MS) is a complex human autoimmune-type inflammatory disease of the central nervous system (CNS). MicroRNA-146a (miR-146a) belongs to an endogenous and non-coding RNA family with 18-22 nucleotides long, which modulates the innate and adaptive immune response.

Methods: Our study aimed to investigate a possible association between rs2910164 and rs2431697 polymorphisms of the miR-146a gene and multiple sclerosis in the Iranian population. A total of 60 MS cases and 100 controls were recruited. Single nucleotide polymorphism (SNP) rs2431697 was genotyped by utilizing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and SNP rs2910164 was genotyped by using Tetra-primer ARMS-PCR. Statistical Analysis conducted by the chi-squared test utilizing SPSS version 21.0 Software. The Hardy-Weinberg equilibrium assumption was evaluated.

Results: The results of the present study suggest the miR-146a gene rs2431697 polymorphism is not associated with multiple sclerosis. However, there is a significant relationship between polymorphism rs2910164 of the miR-146a gene and multiple sclerosis in the population studied (P = 0.012).

Conclusion: Our data provide evidence that the miR-146a gene may be involved in creating the susceptibility to MS in the Iranian population.

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伊朗人群中 miRNA-146a 基因变异与多发性硬化症的关系

背景：多发性硬化症（MS）是一种复杂的人类自身免疫型中枢神经系统（CNS）炎症性疾病。微RNA-146a（miR-146a）属于内源性非编码RNA家族，长度为18-22个核苷酸，可调节先天性和适应性免疫反应：我们的研究旨在调查伊朗人群中 miR-146a 基因 rs2910164 和 rs2431697 多态性与多发性硬化症之间可能存在的关联。研究共招募了 60 例多发性硬化症病例和 100 例对照。利用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）对单核苷酸多态性（SNP）rs2431697进行基因分型，利用四引物ARMS-PCR对SNP rs2910164进行基因分型。统计分析采用 SPSS 21.0 版软件进行卡方检验。对哈迪-温伯格平衡假设进行了评估：本研究结果表明，miR-146a 基因 rs2431697 多态性与多发性硬化症无关。然而，在所研究的人群中，miR-146a 基因 rs2910164 多态性与多发性硬化症有显著关系（P = 0.012）：我们的数据提供了证据，证明 miR-146a 基因可能参与了伊朗人群多发性硬化症易感性的形成。

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