A Lipidomics Approach to Determine the Role of Lipids and Its Crosstalk with Autophagy in Lung Cancer Metastasis.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-03-06 DOI:10.1007/7651_2024_524
Simone C da Silva Rosa, Javad Alizadeh, Rui Vitorino, Arun Surendran, Amir Ravandi, Biniam Kidane, Saeid Ghavami
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Abstract

Non-small cell lung cancer (NSCLC) is among the most malignant tumors with high propensity for metastasis and is the leading cause of cancer-related death globally. Most patients present with regional and distant metastasis, associated with poor prognosis. Lipids may play an essential role in either activating or inhibiting detachment-induced apoptosis (anoikis), where the latter is a crucial mechanism to prevent metastasis, and it may have a cross-talk with autophagy. Autophagy has been shown to be induced in various human cancer metastasis, modulating tumor cell motility and invasion, cancer cell differentiation, resistance to anoikis, and epithelial to mesenchymal transition. Hence, it may play a crucial role in the transition of benign to malignant phenotypes, the core of metastasis initiation. Here, we provide a method we have established in our laboratory for detecting lipids in attached and detached non-small lung cancer cells and show how to analyze lipidomics data to find its correlation with autophagy-related pathways.

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用脂质组学方法确定脂质在肺癌转移中的作用及其与自噬的相互关系
非小细胞肺癌(NSCLC)是转移倾向最高的恶性肿瘤之一,也是全球癌症相关死亡的主要原因。大多数患者会出现区域和远处转移,预后不良。脂质可能在激活或抑制脱落诱导的细胞凋亡(anoikis)中发挥重要作用,而后者是防止转移的关键机制,它可能与自噬存在交叉作用。研究表明,自噬可诱导多种人类癌症转移,调节肿瘤细胞的运动和侵袭、癌细胞分化、抗嗜酸性细胞凋亡以及上皮细胞向间充质细胞的转化。因此,它可能在良性表型向恶性表型的转变过程中起着至关重要的作用,而良性表型向恶性表型的转变是转移发生的核心。在这里,我们提供了一种我们实验室建立的检测附着和脱落的非小肺癌细胞中脂质的方法,并展示了如何分析脂质组学数据以发现其与自噬相关通路的关联。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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