Biocontrol and Growth Promotion Potential of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani that Causes Tobacco Target Spot Disease.

Polish journal of microbiology Pub Date : 2024-03-04 eCollection Date: 2024-03-01 DOI:10.33073/pjm-2024-004
Ning Huang, Xin Jin, Jin-Tao Wen, Yi-Fei Zhang, Xu Yang, Guang-Yu Wei, Yi-Kun Wang, Min Qin
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Abstract

Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the antagonistic effect of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani, its ability to promote the growth of tobacco seedlings, and the expression of disease resistance-related genes for efficient and eco-friendly plant disease control. Our results showed that CTXW 7-6-2 had the most vigorous growth after being cultured for 96 h, and its rate of inhibition of R. solani growth in vitro was 94.02%. The volatile compounds produced by CTXW 7-6-2 inhibited the growth of R. solani significantly (by 96.62%). The fungal growthinhibition rate of the B. subtilis CTXW 7-6-2 broth obtained after high-temperature and no-high-temperature sterile fermentation was low, at 50.88% and 54.63%, respectively. The lipopeptides extracted from the B. subtilis CTXW 7-6-2 fermentation broth showed a 74.88% fungal growth inhibition rate at a concentration of 100 mg/l. Scanning and transmission electron microscopy showed some organelle structural abnormalities, collapse, shrinkage, blurring, and dissolution in the R. solani mycelia. In addition, CTXW 7-6-2 increased tobacco seedling growth and improved leaf and root weight compared to the control. After CTXW 7-6-2 inoculation, tobacco leaves showed the upregulation of the PDF1.2, PPO, and PAL genes, which are closely related to target spot disease resistance. In conclusion, B. subtilis CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.

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枯草芽孢杆菌 CTXW 7-6-2 对烟草靶点病根瘤菌的生物防治和生长促进潜力。
真菌病害会在烟草植株上形成穿孔病斑,导致烟草产量和质量下降。本研究探讨了枯草芽孢杆菌 CTXW 7-6-2 对根肿病的拮抗作用、促进烟苗生长的能力以及抗病相关基因的表达,以实现高效、环保的植物病害控制。结果表明,CTXW 7-6-2 在体外培养 96 小时后生长最为旺盛,其对 R. solani 生长的抑制率为 94.02%。CTXW 7-6-2 产生的挥发性化合物对 R. solani 的生长有明显的抑制作用(96.62%)。高温和非高温无菌发酵后得到的枯草芽孢杆菌 CTXW 7-6-2 肉汤对真菌生长的抑制率较低,分别为 50.88% 和 54.63%。从枯草芽孢杆菌 CTXW 7-6-2 发酵液中提取的脂肽在 100 毫克/升的浓度下对真菌生长的抑制率为 74.88%。扫描和透射电子显微镜显示,R. solani 菌丝体中存在一些细胞器结构异常、塌陷、收缩、模糊和溶解现象。此外,与对照组相比,CTXW 7-6-2 还能促进烟草幼苗的生长,提高叶片和根的重量。接种 CTXW 7-6-2 后,烟草叶片中与靶斑病抗性密切相关的 PDF1.2、PPO 和 PAL 基因上调。总之,枯草芽孢杆菌 CTXW 7-6-2 可能是烟草农业中一种高效的生物防治剂,并能提高植物的生长潜力。
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