Biomass and Functional Diversity of Microbial Communities in Catenas of Reserved and Arable Gray Soils and Chernozems

IF 1.4 4区 农林科学 Q4 SOIL SCIENCE Eurasian Soil Science Pub Date : 2024-03-05 DOI:10.1134/s1064229323602925
K. S. Dushchanova, P. A. Ukrainskiy, N. N. Kashirskaya, T. E. Khomutova, A. V. Borisov
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Abstract

The biomass and functional diversity of microbial communities were studied in the watershed, transit, and accumulative positions in catenas composed of reserved gray soils (Luvic Retic Phaeozems) and chernozems (Haplic Chernozems) of the Belogorye Nature Reserve and arable variants outside the reserve. Microbial biomass was determined by the substrate-induced respiration (SIR) and the content of phospholipids. Multisubstrate testing of respiratory responses was carried out in the MicroResp system after the addition of amino acids, carboxylic acids, and carbohydrates. It was found that microbial biomass decreased in the reserved chernozem from the watershed towards the accumulative part of the slope; minimal values in the gray soil were recorded in the transit part of the catena. It was close in the plowed horizon of agrochernozems in all parts of the catena and 2–3.5 times less than in reserved chernozems. An increase in microbial biomass was recorded in the agrogray soils of the transit and accumulative parts of the catena. Cluster analysis of respiratory responses in the 0–10 and 10–20 cm layers identified two groups of the most demanded substrates. The first group in both layers included citric and ketoglutaric acids, the second group included fructose and succinic acid. Ascorbic acid, sucrose, and glutamine were included in the first group in the 0–10 cm layer and in the second group (along with asparagine and glycine) in the 10–20 cm layer. An increase in metabolic diversity was observed from the watershed to the accumulative position of the catena in all reserved and arable catenas. At the same time, plowing led to its decrease in the 0–10 cm layer: up to 1.5 times in chernozems and up to 4 times in gray soils. In the 10–20 cm layer, similar trend was observed, except for the agrogray soil in the transit part of the catena, where the number of significant responses increased 3.6 times in comparison with the reserved variant.

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保留地和耕地灰土及切尔诺泽姆土壤中微生物群落的生物量和功能多样性
摘要 在别洛戈耶自然保护区的保留灰土(Luvic Retic Phaeozems)和切尔诺泽姆(Haplic Chernozems)以及保护区外的耕地变体组成的鲶鱼群中,研究了分水岭、过境和累积位置的微生物群落的生物量和功能多样性。微生物生物量是通过底物诱导呼吸(SIR)和磷脂含量确定的。在添加氨基酸、羧酸和碳水化合物后,在 MicroResp 系统中进行了多底物呼吸反应测试。研究发现,从分水岭到斜坡的堆积部分,保留的chernozem中的微生物生物量有所下降;在卡特纳河的过境部分,灰土中的微生物生物量值最小。在导管的所有部分,农业耕地中的微生物生物量都很接近,是保留耕地中微生物生物量的 2-3.5 倍。据记录,在导管中段和累积段的农耕土壤中,微生物生物量有所增加。对 0-10 厘米和 10-20 厘米土层的呼吸反应进行聚类分析,确定了两组需求量最大的基质。第一组包括柠檬酸和酮戊二酸,第二组包括果糖和琥珀酸。0-10 厘米层的第一组包括抗坏血酸、蔗糖和谷氨酰胺,10-20 厘米层的第二组(还有天冬酰胺和甘氨酸)包括抗坏血酸、蔗糖和谷氨酰胺。在所有保留地和可耕地的鲶鱼群中,从分水岭到鲶鱼群的累积位置,都能观察到代谢多样性的增加。与此同时,耕地导致 0-10 厘米土层中的代谢多样性减少:在耕地中减少了 1.5 倍,在灰土中减少了 4 倍。在 10-20 厘米土层中,也观察到类似的趋势,但在卡泰纳中转部分的农用灰土中,显著反应的数量比保留变体增加了 3.6 倍。
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来源期刊
Eurasian Soil Science
Eurasian Soil Science 农林科学-土壤科学
CiteScore
2.70
自引率
35.70%
发文量
137
审稿时长
12-24 weeks
期刊介绍: Eurasian Soil Science publishes original research papers on global and regional studies discussing both theoretical and experimental problems of genesis, geography, physics, chemistry, biology, fertility, management, conservation, and remediation of soils. Special sections are devoted to current news in the life of the International and Russian soil science societies and to the history of soil sciences. Since 2000, the journal Agricultural Chemistry, the English version of the journal of the Russian Academy of Sciences Agrokhimiya, has been merged into the journal Eurasian Soil Science and is no longer published as a separate title.
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