Methylation changes of liver DNA during the formation of gallstones.

IF 3 4区 医学 Q2 GENETICS & HEREDITY Epigenomics Pub Date : 2024-03-06 DOI:10.2217/epi-2023-0391
Junbin Peng, Haojie Li, Fang Tong, Jinlong Hu, Min Li, Gan Chen, Dongquan Liu, Jinshan Liu, Rui Wang, Hongyu Xu, Xuanxuan Li, Xinguo Zhong, Jiaming Yao, Baoqiang Cao
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Abstract

Aim: To explore the overall methylation changes in liver tissues during the formation of gallstones, as well as the key pathways and genes involved in the process. Methods: Reduced-representation bisulfite sequencing and RNA sequencing were conducted on the liver tissues of mice with gallstones and control normal mice. Results: A total of 8705 differentially methylated regions in CpG and 1410 differentially expressed genes were identified. The joint analysis indicated that aberrant DNA methylation may be associated with dysregulated gene expression in key pathways such as cholesterol metabolism and bile secretion. Conclusion: We propose for the first time that methylation changes in some key pathway genes in liver tissue may be involved in the formation of gallstones.

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胆结石形成过程中肝脏 DNA 的甲基化变化
目的:探讨胆结石形成过程中肝脏组织的整体甲基化变化,以及参与这一过程的关键通路和基因。方法对胆结石小鼠和正常对照小鼠的肝组织进行还原代表亚硫酸氢盐测序和 RNA 测序。结果共鉴定出 8705 个 CpG 不同甲基化区域和 1410 个不同表达基因。联合分析表明,DNA 甲基化异常可能与胆固醇代谢和胆汁分泌等关键通路的基因表达失调有关。结论:我们首次提出,肝组织中一些关键通路基因的甲基化变化可能与胆结石的形成有关。
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来源期刊
Epigenomics
Epigenomics GENETICS & HEREDITY-
CiteScore
5.80
自引率
2.60%
发文量
95
审稿时长
>12 weeks
期刊介绍: Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
期刊最新文献
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