Anti-Trichomonas vaginalis Activity of Marine Ascidians (Tunicates; Ascidiacea) from the Bushehr Province, Iran

Q3 Medicine Turkiye parazitolojii dergisi Pub Date : 2024-03-05 DOI:10.4274/tpd.galenos.2023.96658

Hamideh Malekhayati, Afshar Bargahi, Soleiman Khorami, Mohsen Khataminejad, Moradali Fouladvand

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Abstract

Objective: The aim of the current research is to evaluate the antiparasite effects of compounds isolated from marine ascidian tunicates on Trichomonas vaginalis.

Methods: Ascidian tunicates after collection were cut into small pieces, freeze-dried, and powdered. The resulting material was subjected to extraction in double-distilled water, ethanol, n-hexane, and dichloromethane. To fractionate the extracts and identify the most bioactive compound, silica gel column chromatography and GC-M/S analysis were used.

Results: Fraction 18 of silica gel column chromatography of ethanol extract was the most effective against T. vaginalis. The respective IC₅₀, CC₅₀, and SI values for fraction 18 were 28.62 μg/mL, ˃800 μg/mL, and ˃27.95. GC-M/S analysis of this fraction identified a major phenolic compound (2, 4-bis (1, 1-dimethyl ethyl), whose toxicity against vero cells was only 10.15%.

Conclusion: The ethanolic fraction containing phenol-2,4-bis (1,1-dimethylethyl), which has a potent lethality effect on T. vaginalis, may be considered as an antiparasite drug candidate.

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伊朗布什尔省海洋无脊椎动物（鳞目；无脊椎动物科）的抗阴道毛滴虫活性。

研究目的本研究的目的是评估从海洋腹腔栉水母中分离出来的化合物对阴道毛滴虫的抗寄生虫作用：方法：将采集的海生鳞片贝切成小块，冷冻干燥并制成粉末。所得材料在双蒸水、乙醇、正己烷和二氯甲烷中进行萃取。为了对提取物进行分馏，并确定生物活性最强的化合物，采用了硅胶柱色谱法和气相色谱-质谱-串联质谱分析法：结果：乙醇提取物的硅胶柱层析馏分 18 对阴道球菌最有效。馏分 18 的 IC50、CC50 和 SI 值分别为 28.62 μg/mL、˃800 μg/mL 和 ˃27.95。对该馏分的 GC-M/S 分析确定了一种主要的酚类化合物（2，4-双（1，1-二甲基乙基），其对vero 细胞的毒性仅为 10.15%：含有苯酚-2,4-双（1,1-二甲基乙基）的乙醇馏分对阴道茨菰有很强的杀灭作用，可作为抗寄生虫药物候选物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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