The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy

IF 3.5 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Structural Biology: X Pub Date : 2024-03-06 DOI:10.1016/j.yjsbx.2024.100099
Sharmila Fiama das Neves Ortiz , Raphael Verdan , Gustavo Miranda Rocha , Kildare Miranda , Marlene Benchimol
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Abstract

Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted infection worldwide, with an estimated 260 million new cases annually. T. vaginalis contains organelles common to all eukaryotic cells, uncommon cell structures such as hydrogenosomes, and a complex and elaborate cytoskeleton constituting the mastigont system. The mastigont system is mainly formed by several proteinaceous structures associated with basal bodies, the pelta-axostylar complex made of microtubules, and striated filaments named the costa and the parabasal filaments (PFs). Although the structural organization of trichomonad cytoskeletons has been analyzed using several techniques, observation using a new generation of scanning electron microscopes with a resolution exceeding 1 nm has allowed more detailed visualization of the three-dimensional organization of the mastigont system. In this study, we have investigated the cytoskeleton of T. vaginalis using a diverse range of scanning probe microscopy techniques, which were complemented by electron tomography and Fast-Fourier methods. This multi-modal approach has allowed us to characterize an unknown parabasal filament and reveal the ultrastructure of other striated fibers that have not been published before. Here, we show the differences in origin, striation pattern, size, localization, and additional details of the PFs, thus improving the knowledge of the cell biology of this parasite.

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阴道毛滴虫的副基质丝:一种新的丝状物和使用 0.8 纳米分辨率扫描电子显微镜的观察结果
阴道毛滴虫是滴虫病的病原体,是全球最常见的非病毒性传播感染,估计每年新增病例 2.6 亿例。阴道毛滴虫含有所有真核细胞共有的细胞器、不常见的细胞结构(如氢小体)以及复杂而精细的细胞骨架,这些构成了阴道毛滴虫系统(mastigont system)。mastigont系统主要由与基底体、由微管组成的骨盆-轴柱复合体以及名为costa和parabasal丝(PFs)的横纹丝相关的几种蛋白质结构组成。虽然已经使用多种技术分析了毛滴虫细胞骨架的结构组织,但使用分辨率超过 1 nm 的新一代扫描电子显微镜进行观察,可以更详细地观察到丝束系统的三维组织。在这项研究中,我们使用了多种扫描探针显微镜技术,并辅以电子断层扫描和快速傅立叶方法,对阴道蓟马的细胞骨架进行了研究。这种多模式方法使我们得以描述一种未知副基丝的特征,并揭示了以前未发表过的其他横纹纤维的超微结构。在这里,我们展示了副寄生虫丝在起源、纹路模式、大小、定位和其他细节方面的差异,从而增进了对这种寄生虫细胞生物学的了解。
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来源期刊
Journal of Structural Biology: X
Journal of Structural Biology: X Biochemistry, Genetics and Molecular Biology-Structural Biology
CiteScore
6.50
自引率
0.00%
发文量
20
审稿时长
62 days
期刊最新文献
Corrigendum to “Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography” [J. Struct. Biol.: X 10(2024) 100113] Editorial by Natalie Reznikov [for Buss et al., “Hierarchical organization of bone in three dimensions: A twist of twists” (2022)] Structural analysis of the stable form of fibroblast growth factor 2 – FGF2-STAB Localization of albumin with correlative super resolution light- and electron microscopy in the kidney Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography
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