Lily Parkinson, Henrike Kuder, Alexandra Kehl, Amie Koenig, Jennifer Good, Jennifer Hausmann, Urs Giger
{"title":"NONDOMESTIC FELID <i>ABC</i> BLOOD PHENOTYPING, GENOTYPING, AND CROSSMATCHING.","authors":"Lily Parkinson, Henrike Kuder, Alexandra Kehl, Amie Koenig, Jennifer Good, Jennifer Hausmann, Urs Giger","doi":"10.1638/2023-0025","DOIUrl":null,"url":null,"abstract":"<p><p>Based upon previous clinical experience with domestic cats (<i>Felis catus</i>), the ability to assess <i>ABC</i> blood types and blood (in-)compatibilities of nondomestic felids, and adequately consider and plan for blood transfusions, may be important. Although nondomestic felids appear to have an <i>ABC</i> blood group system similar to domestic cats, typing with point-of-care kits and by <i>CMAH</i> genotyping for domestic cats have not been reported. In this study, 162 blood samples from 18 different nondomestic felid species (cheetah [<i>Acinonyx jubatus</i>, <i>n</i> = 42], lion [<i>Panthera leo</i>, <i>n</i> = 33], tiger [<i>Panthera tigris</i>, <i>n</i> = 23], Canada lynx [<i>Lynx canadensis</i>, <i>n</i> = 11], snow leopard [<i>Uncia uncia</i>, <i>n</i> = 10], puma [<i>Puma concolor</i>, <i>n</i> = 7], clouded leopard [<i>Neofelis nebulosa</i>, <i>n</i> = 6], serval [<i>Leptailurus serval</i>, <i>n</i> = 5], jaguar [<i>Panthera onca</i>, <i>n</i> = 5], fishing cat [<i>Prionailurus viverrinus</i>, <i>n</i> = 4], Pallas cat [<i>Felis manul</i>, <i>n</i> = 3], bobcat [<i>Lynx rufus</i>, <i>n</i> = 3], ocelot [<i>Leopardus pardalis</i>, <i>n</i> = 3], black footed cat [<i>Felis nigripes</i>, <i>n</i> = 2], leopard [<i>Panthera pardus</i>, <i>n</i> = 2], African wildcat [<i>Felis lybica</i>, <i>n</i> = 1], caracal [<i>Caracal caracal</i>, <i>n</i> = 1], and sand cat [<i>Felis margarita</i>, <i>n</i> = 1]) were <i>ABC</i> blood typed by laboratory and point-of-care tests, genotyped for four known <i>CMAH</i> variants for type <i>B</i> and type <i>C</i> (<i>AB</i>) phenotypes, and crossmatched with one another and domestic type <i>A</i> cats. Traditional tube typing identified blood type <i>A</i> (<i>n</i> = 106), type <i>B</i> (<i>n</i> = 8), type <i>C</i> (<i>n</i> = 43), and no discernible <i>ABC</i> type (<i>n</i> = 4). Several discrepancies were found between point-of-care and traditional typing test results. None of the tested felids possessed the four CMAH variants responsible for type <i>B</i> and <i>C</i> (<i>AB</i>) in domestic cats. Crossmatch incompatibilities (≥2+ agglutination) were identified within and between nondomestic felid species and beyond <i>ABC</i> incompatibilities. Of 26 crossmatches performed between domestic cats and various nondomestic felids, only 7 (27%) were compatible. In conclusion, point-of-care typing kits and <i>CMAH</i> genotyping, successfully used in domestic cats, may not identify the correct <i>ABC</i> blood type in nondomestic felids. Prior crossmatching is recommended to increase the likelihood of compatible transfusions between any nondomestic felids.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":"55 1","pages":"143-154"},"PeriodicalIF":0.7000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Zoo and Wildlife Medicine","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1638/2023-0025","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Based upon previous clinical experience with domestic cats (Felis catus), the ability to assess ABC blood types and blood (in-)compatibilities of nondomestic felids, and adequately consider and plan for blood transfusions, may be important. Although nondomestic felids appear to have an ABC blood group system similar to domestic cats, typing with point-of-care kits and by CMAH genotyping for domestic cats have not been reported. In this study, 162 blood samples from 18 different nondomestic felid species (cheetah [Acinonyx jubatus, n = 42], lion [Panthera leo, n = 33], tiger [Panthera tigris, n = 23], Canada lynx [Lynx canadensis, n = 11], snow leopard [Uncia uncia, n = 10], puma [Puma concolor, n = 7], clouded leopard [Neofelis nebulosa, n = 6], serval [Leptailurus serval, n = 5], jaguar [Panthera onca, n = 5], fishing cat [Prionailurus viverrinus, n = 4], Pallas cat [Felis manul, n = 3], bobcat [Lynx rufus, n = 3], ocelot [Leopardus pardalis, n = 3], black footed cat [Felis nigripes, n = 2], leopard [Panthera pardus, n = 2], African wildcat [Felis lybica, n = 1], caracal [Caracal caracal, n = 1], and sand cat [Felis margarita, n = 1]) were ABC blood typed by laboratory and point-of-care tests, genotyped for four known CMAH variants for type B and type C (AB) phenotypes, and crossmatched with one another and domestic type A cats. Traditional tube typing identified blood type A (n = 106), type B (n = 8), type C (n = 43), and no discernible ABC type (n = 4). Several discrepancies were found between point-of-care and traditional typing test results. None of the tested felids possessed the four CMAH variants responsible for type B and C (AB) in domestic cats. Crossmatch incompatibilities (≥2+ agglutination) were identified within and between nondomestic felid species and beyond ABC incompatibilities. Of 26 crossmatches performed between domestic cats and various nondomestic felids, only 7 (27%) were compatible. In conclusion, point-of-care typing kits and CMAH genotyping, successfully used in domestic cats, may not identify the correct ABC blood type in nondomestic felids. Prior crossmatching is recommended to increase the likelihood of compatible transfusions between any nondomestic felids.
期刊介绍:
The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers.
The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution.
Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.