DEVELOPMENT OF A HEMOLYMPH COLLECTION TECHNIQUE AND REPORT OF BIOCHEMICAL PARAMETERS OF HEMOLYMPH IN A MANAGED POPULATION OF FEMALE THORNY DEVIL STICK INSECTS (EURYCANTHA CALCARATA).

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Journal of Zoo and Wildlife Medicine Pub Date : 2024-03-01 DOI:10.1638/2023-0063
Melinda A Gorges, Julie A Balko, Andrew W Lathan, Taylor M Gregory, Ashlyn C Heniff, Gregory A Lewbart
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Abstract

Insects are increasingly common in households, zoological collections, research, and food industries. Increased knowledge of insect health parameters is necessary to ensure proper welfare. The study goal was to develop a hemolymph collection technique and report hemolymph serum biochemical parameters for the thorny devil stick insect (Eurycantha calcarata). Clinically healthy adult stick insects (5 males and 14 females, 15.1-24.7 g) were enrolled. Four collection techniques were evaluated. Hemolymph collection was unsuccessful in males, but was successful in females by using a single technique. The insect was manually restrained in an elevated position and an 18-ga × 2.54-cm needle was used to puncture the membrane just caudal to the third pair of legs. With the puncture site directed ventrally, ≤1 ml of hemolymph was collected via gravity and allowed to visibly clot. The sample was then centrifuged, and the serum was separated. Serum samples were individually analyzed (Avian/Reptilian Profile Plus, VetScan VS2, Abaxis, Inc, Union City, CA 94587, USA). Fourteen samples (0.2-1.0 ml) were collected from 14 females. Median (minimum-maximum) parameters included the following: aspartate aminotransferase 12 (0-45) U/L, creatinine kinase 25 (0-76) U/L, uric acid 7.5 (3.1-13.7) mg/dl, glucose 12 (8-22) mg/dl, calcium 18.6 (17.2-19.4) mg/dl, phosphorus 15.0 (n = 1) or >30.0 (n = 13) mg/dL, total protein 2.7 (1.6-2.9) g/dL, albumin 0.9 (0.2-1.2) g/dL, globulin 1.7 (1.6-1.8) g/dL (n = 6) or not quantified (n = 8), potassium 10.6 (9.0-11.8) mmol/L, sodium < 100 mmol/L, and bile acids 0 lmol/L. This is the first report of biochemistry parameters in clinically healthy female stick insects. Larger sample sizes are needed to establish statistically valid reference ranges. Hemolymph collection techniques for male stick insects warrant further investigation.

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开发一种血淋巴采集技术,并报告雌性荆条魔棒虫(eurycantha calcarata)管理种群的血淋巴生化参数。
昆虫在家庭、动物收藏、研究和食品工业中越来越常见。增加对昆虫健康参数的了解是确保适当福利的必要条件。本研究的目标是开发一种血淋巴采集技术,并报告荆条魔棒昆虫(Eurycantha calcarata)的血淋巴血清生化参数。研究对象为临床健康的成年粘虫(5 雄 14 雌,体重 15.1-24.7 克)。对四种采集技术进行了评估。雄性昆虫的血淋巴采集不成功,但雌性昆虫的血淋巴采集采用了单一技术。人工将昆虫置于高位,用 18 ga × 2.54 厘米的针头刺穿第三对腿尾部的薄膜。穿刺部位朝向腹侧,通过重力收集≤1毫升的血淋巴,让其明显凝结。然后将样本离心,分离血清。分别对血清样本进行分析(Avian/Reptilian Profile Plus, VetScan VS2, Abaxis, Inc, Union City, CA 94587, USA)。从 14 只雌蛙身上采集了 14 份样本(0.2-1.0 毫升)。中位数(最小值-最大值)参数包括:天冬氨酸氨基转移酶 12 (0-45) U/L、肌酸激酶 25 (0-76) U/L、尿酸 7.5 (3.1-13.7) mg/dl、葡萄糖 12 (8-22) mg/dl、钙 18.6 (17.2-19.4) mg/dl、磷 15.总蛋白 2.7 (1.6-2.9) g/dL,白蛋白 0.9 (0.2-1.2) g/dL,球蛋白 1.7 (1.6-1.8) g/dL(n = 6)或未定量(n = 8),钾 10.6 (9.0-11.8) mmol/L,钠 < 100 mmol/L,胆汁酸 0 lmol/L。这是首次报告临床健康雌性粘虫的生化参数。需要更大的样本量才能确定统计学上有效的参考范围。雄性粘虫的血液淋巴采集技术值得进一步研究。
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来源期刊
Journal of Zoo and Wildlife Medicine
Journal of Zoo and Wildlife Medicine 农林科学-兽医学
CiteScore
1.70
自引率
14.30%
发文量
74
审稿时长
9-24 weeks
期刊介绍: The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers. The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution. Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.
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