Multidrug-Resistant and Carbapenemase Gene-Carrying Acinetobacter colistiniresistens Isolated from Bloodstream Infection.

IF 2.3 4区医学 Q3 INFECTIOUS DISEASES Microbial drug resistance Pub Date : 2024-05-01 Epub Date: 2024-03-07 DOI:10.1089/mdr.2023.0083

Hae-Sun Chung, Chorong Hahm, Min-Kyung So, Miae Lee

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Abstract

In this study, we investigated the antimicrobial susceptibility and molecular characteristics of antimicrobial resistance of Acinetobacter colistiniresistens strains isolated from the bloodstream using whole-genome sequencing. Clinical isolates identified as Acinetobacter baumannii and showing colistin resistance at the time of detection were collected. Antimicrobial susceptibility was determined using the VITEK2 system (bioMérieux) and Sensititre system (Thermo Fisher Scientific). Species identification and antimicrobial resistance gene searches were performed through whole-genome sequencing. Through whole-genome sequencing, three colistin-resistant strains from the bloodstream were identified as A. colistiniresistens. All three A. colistiniresistens strains were resistant to two or more antimicrobial agents except for colistin, and two of them were resistant to carbapenems. Genes involved in aminoglycoside [AAC(3)-Ⅱb, AAC(6')-Ⅰj, aadA2, ANT(3″)-Ⅱb, APH(3')-Ⅵa], macrolide (mphD, msrE), carbapenem and cephalosporin (OXA-420, VIM-2), fluoroquinolone and tetracycline (adeF), and sulfonamide (sul1, sul2) resistance were detected. We report multidrug-resistant A. colistiniresistens strains isolated from the bloodstream through whole-genome sequencing. Two strains carried carbapenemase genes, and this is the first report of VIM-2-producing A. colistiniresistens.

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从血流感染中分离出的耐多药和携带碳青霉烯酶基因的大肠埃希菌。

在本研究中，我们利用全基因组测序技术研究了从血液中分离出的大肠埃希菌菌株的抗菌药敏感性和耐药性分子特征。收集被鉴定为鲍曼不动杆菌并在检测时显示出耐药的临床分离株。使用 VITEK2 系统（生物梅里埃公司）和 Sensititre 系统（赛默飞世尔科技公司）测定抗菌药敏感性。通过全基因组测序进行菌种鉴定和抗菌药耐药性基因搜索。通过全基因组测序，从血液中鉴定出三株耐大肠杆菌素的菌株为大肠杆菌（A. colistiniresistens）。除秋水仙素外，这三株大肠杆菌均对两种或两种以上的抗菌药产生耐药性，其中两株对碳青霉烯类产生耐药性。涉及氨基糖苷类[AAC(3)-Ⅱb、AAC(6')-Ⅰj、aadA2、ANT(3″)-Ⅱb、APH(3')-Ⅵa]、大环内酯类（mphD、msrE）的基因、我们还检测到对碳青霉烯类和头孢菌素类（OXA-420、VIM-2）、氟喹诺酮类和四环素类（adeF）以及磺胺类（sul1、sul2）的耐药性。我们报告了通过全基因组测序从血液中分离出的耐多药大肠杆菌菌株。两株菌株携带碳青霉烯酶基因，这是首次报道产VIM-2的大肠杆菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial drug resistance 医学-传染病学

CiteScore

6.00

自引率

3.80%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.