Dongmei Shi, Zhiya Yang, Wanqing Liao, Chen Liu, Liang Zhao, Huilin Su, Xiaodong Wang, Huan Mei, Min Chen, Yinggai Song, Sybren de Hoog, Shuwen Deng
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引用次数: 0
Abstract
Background: Chromoblastomycosis is the World Health Organization (WHO)-recognized fungal implantation disease that eventually leads to severe mutilation. Cladophialophora carrionii (C. carrionii) is one of the agents. However, the pathogenesis of C. carrionii is not fully investigated yet.
Methods: We investigated the pathogenic potential of the fungus in a Galleria mellonella (G. mellonella) larvae infection model. Six strains of C. carrionii, and three of its environmental relative C. yegresii were tested. The G. mellonella model was also applied to determine antifungal efficacy of amphotericin B, itraconazole, voriconazole, posaconazole, and terbinafine.
Results: All strains were able to infect the larvae, but virulence potentials were strain-specific and showed no correlation with clinical background of the respective isolate. Survival of larvae also varied with infection dose, and with growth speed and melanization of the fungus. Posaconazole and voriconazole exhibited best activity against Cladophialophora, followed by itraconazole and terbinafine, while limited efficacy was seen for amphotericin B.
Conclusion: Infection behavior deviates significantly between strains. In vitro antifungal susceptibility of tested strains only partly explained the limited treatment efficacy in vivo.
背景:着色真菌病是世界卫生组织(WHO)公认的真菌植入性疾病,最终会导致严重的肢体残缺。腐肉癣菌(Cladophialophora carrionii,C. carrionii)是病原体之一。然而,C. carrionii 的致病机理尚未得到充分研究:方法:我们研究了该真菌在麦角菌(G. mellonella)幼虫感染模型中的致病潜力。我们测试了六株 C. carrionii 真菌及其三株环境亲缘 C. yegresii 真菌。此外,还采用了G. mellonella模型来确定两性霉素B、伊曲康唑、伏立康唑、泊沙康唑和特比萘芬的抗真菌效果:结果:所有菌株都能感染幼虫,但毒力潜能是菌株特异性的,与各自分离株的临床背景无关。幼虫的存活率也随感染剂量、生长速度和真菌黑化程度而变化。泊沙康唑和伏立康唑对栉水母的活性最好,其次是伊曲康唑和特比萘芬,而两性霉素 B 的效果有限:结论:不同菌株的感染行为差异很大。测试菌株的体外抗真菌敏感性只能部分解释体内治疗效果有限的原因。
Ima FungusAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍:
The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue