YBX-1 alleviates sepsis-stimulated lung epithelial cell injury.

IF 2.5 4区医学 Q3 ALLERGY Allergologia et immunopathologia Pub Date : 2024-03-01 eCollection Date: 2024-01-01 DOI:10.15586/aei.v52i2.1068

Xin Lu, Shouqian Dai, Pengfei Li, Yuqian Zhou, Feng Xu

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Abstract

Objective: To explore the role of Y-box binding protein 1 (YBX-1) in the lipopolysaccharide (LPS)-stimulated inflammation and oxidative stress of BEAS-2B cell line and clarify the underlying mechanism.

Methods: LPS-stimulated BEAS-2B cells were used as a cell model of sepsis-stimulated acute lung injury (ALI). Immunoblot and quantitative polymerase chain reaction assays were used to detect the expression of YBX-1 in LPS-stimulated BEAS-2B cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, TdT-mediated dUTP nick end labeling, and immunoblot assays were conducted to determine the effects of YBX-1 on cell survival. JC-1 staining and adenosine triphosphate production were used to detect the effects of YBX-1 on mitochondrial function. Immunostaining and enzyme-linked immunosorbent serologic assay were performed to examine the effects of YBX-1 on the inflammation and oxidative stress of cells. Immunoblot assay was conducted to confirm the mechanism.

Results: YBX-1 was lowly expressed in LPS-stimulated BEAS-2B cells and enhanced the survival of LPS-stimulated lung epithelial cells. In addition, YBX-1 improved mitochondrial function of LPS-stimulated BEAS-2B cells. YBX-1 inhibited the inflammation and oxidative stress of LPS-stimulated BEAS-2B cells. Mechanically, YBX-1 inhibited mitogen-activated protein kinase (MAPK) axis, thereby alleviating sepsis-stimulated ALI.

Conclusion: YBX-1 alleviated inflammation and oxidative stress of LPS-stimulated BEAS-2B cells via MAPK axis.

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YBX-1 可减轻败血症刺激的肺上皮细胞损伤

目的探讨Y-盒结合蛋白1（YBX-1）在脂多糖（LPS）刺激的BEAS-2B细胞系炎症和氧化应激中的作用，并阐明其潜在机制：方法：以LPS刺激的BEAS-2B细胞作为脓毒症刺激的急性肺损伤（ALI）细胞模型。免疫印迹和定量聚合酶链反应检测 YBX-1 在 LPS 刺激的 BEAS-2B 细胞中的表达。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-溴化四氮唑、TdT 介导的 dUTP 缺口标记和免疫印迹检测来确定 YBX-1 对细胞存活的影响。JC-1 染色和三磷酸腺苷生成被用来检测 YBX-1 对线粒体功能的影响。免疫染色法和酶联免疫吸附血清法检测了 YBX-1 对细胞炎症和氧化应激的影响。免疫印迹分析证实了这一机制：结果：YBX-1在LPS刺激的BEAS-2B细胞中低表达，并能提高LPS刺激的肺上皮细胞的存活率。此外，YBX-1 还能改善 LPS 刺激的 BEAS-2B 细胞的线粒体功能。YBX-1 可抑制 LPS 刺激的 BEAS-2B 细胞的炎症和氧化应激。从机制上讲，YBX-1 可抑制丝裂原活化蛋白激酶（MAPK）轴，从而缓解败血症刺激的 ALI：结论：YBX-1 可通过 MAPK 轴减轻 LPS 刺激的 BEAS-2B 细胞的炎症和氧化应激反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.