Rosemary essential oil potentiates the antitumour activity of 5-fluorouracil in human colorectal carcinoma cells.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY Journal of Pharmacy and Pharmacology Pub Date : 2024-06-06 DOI:10.1093/jpp/rgae022

Amine Ali El Sala, Ghada Khawaja, Mahmoud Khalil, Sherine Abdel Salam

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Abstract

Objectives: Improving response rates in colorectal cancer (CRC) is an urgent clinical need. This study aimed to explore the synergistic action of Lebanese rosemary essential oil (REO) and 5-fluorouracil (5-FU) in HCT116 CRC cells.

Methods: We tested the cell viability of monotherapy and combination therapy. The combination index was calculated using CompuSyn software to evaluate drug-drug interactions and the level of synergistic cytotoxicity. We also evaluated cell migration and cytopathology. Furthermore, cell apoptosis-related proteins (i.e. Bax and Bcl-2) were measured by Western blot analysis.

Key findings: The REO/5-FU combination synergistically reduced cell viability, effectively decreased cell migration, and increased the Bax/Bcl-2 ratio in HCT116 cells. This triggered a proapoptotic morphology and initiated an apoptotic cascade in HCT116 cells, as indicated by a higher Bax/Bcl-2 ratio.

Conclusions: Our results provide evidence of the REO/5-FU combination as a better approach to improve 5-FU anticancer efficacy and allow the use of lower 5-FU doses due to the adjuvant effect of REO.

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迷迭香精油能增强 5-氟尿嘧啶对人类结直肠癌细胞的抗肿瘤活性。

目的：提高结直肠癌（CRC）的应答率是临床的迫切需要。本研究旨在探讨黎巴嫩迷迭香精油（REO）和 5-氟尿嘧啶（5-FU）在 HCT116 CRC 细胞中的协同作用：方法：我们测试了单一疗法和联合疗法的细胞活力。方法：我们测试了单药治疗和联合治疗的细胞活力，并使用 CompuSyn 软件计算了联合指数，以评估药物间的相互作用和协同细胞毒性水平。我们还评估了细胞迁移和细胞病理学。此外，细胞凋亡相关蛋白（即 Bax 和 Bcl-2）也通过 Western 印迹分析进行了测定：主要发现：REO/5-FU 组合可协同降低 HCT116 细胞的细胞活力，有效减少细胞迁移，并提高 Bax/Bcl-2 比率。这在 HCT116 细胞中引发了促凋亡形态，并启动了细胞凋亡级联，Bax/Bcl-2 比值升高就表明了这一点：我们的研究结果证明，REO/5-FU 组合是提高 5-FU 抗癌疗效的更好方法，并且由于 REO 的辅助作用，可以使用较低的 5-FU 剂量。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.