Maize catalases are recruited by a virus to modulate viral multiplication and infection.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-03-01 DOI:10.1111/mpp.13440
Yiying Tian, Zhiyuan Jiao, Fangfang Qi, Wendi Ma, Yuming Hao, Xinyu Wang, Liyang Xie, Tao Zhou, Zaifeng Fan
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Abstract

Given the detrimental effects of excessive reactive oxygen species (ROS) accumulation in plant cells, various antioxidant mechanisms have evolved to maintain cellular redox homeostasis, encompassing both enzymatic components (e.g., catalase, superoxide dismutase) and non-enzymatic ones. Despite extensive research on the role of antioxidant systems in plant physiology and responses to abiotic stresses, the potential exploitation of antioxidant enzymes by plant viruses to facilitate viral infection remains insufficiently addressed. Herein, we demonstrate that maize catalases (ZmCATs) exhibited up-regulated enzymatic activities upon sugarcane mosaic virus (SCMV) infection. ZmCATs played crucial roles in SCMV multiplication and infection by catalysing the decomposition of excess cellular H2 O2 and promoting the accumulation of viral replication-related cylindrical inclusion (CI) protein through interaction. Peroxisome-localized ZmCATs were found to be distributed around SCMV replication vesicles in Nicotiana benthamiana leaves. Additionally, the helper component-protease (HC-Pro) of SCMV interacted with ZmCATs and enhanced catalase activities to promote viral accumulation. This study unveils a significant involvement of maize catalases in modulating SCMV multiplication and infection through interaction with two viral factors, thereby enhancing our understanding regarding viral strategies for manipulating host antioxidant mechanisms towards robust viral accumulation.

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玉米过氧化氢酶被病毒招募来调节病毒的繁殖和感染。
鉴于活性氧(ROS)在植物细胞中积累过多会产生有害影响,因此进化出了各种抗氧化机制来维持细胞的氧化还原平衡,其中包括酶促成分(如过氧化氢酶、超氧化物歧化酶)和非酶促成分。尽管对抗氧化系统在植物生理和非生物胁迫响应中的作用进行了广泛的研究,但对植物病毒利用抗氧化酶促进病毒感染的可能性仍然缺乏足够的研究。在本文中,我们证明了玉米过氧化氢酶(ZmCATs)在甘蔗花叶病毒(SCMV)感染时表现出上调的酶活性。ZmCATs 通过催化分解细胞中过量的 H2 O2,并通过相互作用促进与病毒复制相关的圆柱包涵体(CI)蛋白的积累,从而在 SCMV 的繁殖和感染过程中发挥关键作用。研究发现,过氧化物酶体定位的 ZmCATs 分布在烟草叶片中 SCMV 复制囊泡周围。此外,SCMV 的辅助成分蛋白酶(HC-Pro)与 ZmCATs 相互作用,增强了过氧化氢酶的活性,从而促进了病毒的积累。这项研究揭示了玉米过氧化氢酶通过与两种病毒因子相互作用,在调节 SCMV 的繁殖和感染过程中的重要作用,从而加深了我们对病毒操纵宿主抗氧化机制以实现强大病毒积累的策略的理解。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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