Mutations associated with boscalid and pyraclostrobin resistance of Botrytis cinerea from vegetable fields in Turkey

IF 2.5 3区农林科学 Q2 Agricultural and Biological Sciences Tropical Plant Pathology Pub Date : 2024-03-08 DOI:10.1007/s40858-024-00646-4

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Abstract

Botrytis cinerea Pers. is a polyphagous fungal pathogen that can cause significant damage in the field, warehouse, and greenhouse conditions. In Turkey, fungicides with site-specific modes containing the active ingredients boscalid and pyraclostrobin are used to control B. cinerea. In this study, it was aimed to determine the mutations associated with resistance to boscalid and pyraclostrobin active substances in B. cinerea isolates obtained from tomato, pepper, eggplant, and lettuce in Turkey. In the in vitro bioassay, a conidial germination test was performed. It was determined that 26% of the isolates used in the bioassay test were resistant. After that, mutations associated with resistance were investigated. Mutations associated with boscalid resistance were found in 18 isolates whose SdhB gene was sequenced. No mutations were detected in sensitive MH51 and Ant34 isolates. It has been determined that N230I and H272R mutations were found to be frequent in B. cinerea populations in Turkey. P225F mutation was detected only in the B4 isolate obtained from the pepper in Antalya. The mutations associated with boscalid resistance detected in this study are reported for the first time in Turkey. The G143A mutation associated with pyraclostrobin resistance was detected in all the isolates sequenced.

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土耳其菜地中与啶虫脒和吡唑醚菌酯抗性有关的突变

摘要灰霉病菌（Botrytis cinerea Pers.）是一种多食性真菌病原体，可在田间、仓库和温室条件下造成严重危害。在土耳其，含有啶虫脒和吡唑醚菌酯活性成分的杀菌剂被用于控制灰葡萄孢菌。本研究旨在确定土耳其番茄、辣椒、茄子和莴苣中分离出的菌株对硼啶醇和吡唑醚菌酯活性物质产生抗性的相关突变。在体外生物测定中，进行了分生孢子萌发试验。结果表明，在生物测定试验中使用的分离物中有 26% 具有抗药性。随后，对与抗性相关的突变进行了调查。在对 SdhB 基因进行测序的 18 个分离物中发现了与 Boscalid 抗性有关的突变。在敏感的 MH51 和 Ant34 分离物中没有发现突变。经确定，土耳其的 B. cinerea 群体中经常出现 N230I 和 H272R 突变。只有从安塔利亚辣椒中获得的 B4 分离物中发现了 P225F 突变。本研究中发现的与硼沙利抗性有关的突变在土耳其还是首次报道。在所有测序的分离物中都检测到了与吡唑醚菌酯抗性有关的 G143A 突变。

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来源期刊

Tropical Plant Pathology PLANT SCIENCES-

CiteScore

4.50

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.