Hydrocortisone UV-Vis Spectrophotometric Study: Stability, Determination of Its Acidity Constants and Quantification

IF 0.8 4区 化学 Q4 SPECTROSCOPY Journal of Applied Spectroscopy Pub Date : 2024-03-08 DOI:10.1007/s10812-024-01709-9
G. M. Ramos-Reyes, S. Corona-Avendaño, M. T. Ramírez-Silva, M. Romero-Romo, M. Palomar-Pardavé
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Abstract

Hydrocortisone is a glucocorticoid-type hormone produced naturally by the organism, such that it maintains the body functional during physical or mental stress episodes. The present work focuses on the UV-Vis spectrophotometric assessment of the chemical stability of hydrocortisone in an aqueous medium during the time of analysis, determination of its acidity constants through a robust methodology that involves the usage of SQUAD software and validation of an analytical method to determine hydrocortisone by means of UV-Vis spectrophotometry, in pharmaceuticals. The acidity constant was determined as pKa 11.45 ± 0.02 (298 K), and the molar absorptivity coefficients and the molar absorptivity coefficients of the species involved in this acidity equilibrium were also determined as a function of the wavelength. The analytical method developed attained detection and quantification limits of 386 ± 3 and 1280 ± 10 nM, respectively, whereas the validation allows reliable quantification of hydrocortisone in pharmaceuticals.

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氢化可的松紫外可见分光光度法研究:稳定性、酸度常数的测定和定量
氢化可的松是机体自然产生的一种糖皮质激素,在身体或精神紧张时可维持机体功能。本研究的重点是用紫外可见分光光度法评估氢化可的松在水介质中的化学稳定性,通过使用 SQUAD 软件的可靠方法确定其酸度常数,并验证用紫外可见分光光度法测定药品中氢化可的松的分析方法。测定的酸度常数为 pKa 11.45 ± 0.02 (298 K),还测定了参与酸度平衡的物种的摩尔吸收系数和摩尔吸收系数与波长的函数关系。所开发的分析方法的检出限和定量限分别为 386 ± 3 nM 和 1280 ± 10 nM,通过验证可对药物中的氢化可的松进行可靠的定量。
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来源期刊
CiteScore
1.30
自引率
14.30%
发文量
145
审稿时长
2.5 months
期刊介绍: Journal of Applied Spectroscopy reports on many key applications of spectroscopy in chemistry, physics, metallurgy, and biology. An increasing number of papers focus on the theory of lasers, as well as the tremendous potential for the practical applications of lasers in numerous fields and industries.
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