Development and Validation of the UV-Spectrophotometric Method for the Determination of Cordycepin, a Nucleoside

Abstract

The primary, bioactive component of Cordyceps militaris, known as cordycepin (3'-deoxyadenosine), has been utilized extensively as a traditional medicinal ingredient and a nutritious diet in Asian nations. To determine the amount of cordycepin in the C. militaris, high-performance liquid chromatography (HPLC) is shown to be an effective, sensitive, and straightforward analytical technique. Other methods for estimating cordycepin include near infrared spectroscopy and capillary electrophoresis–mass spectrometry. The UV-Vis spectroscopy approach was used to quantify cordycepin, and the results were statistically confirmed and compared with an HPLC method. For linearity, repeatability, precision, reproducibility, limit of detection and limit of quantification, the method was verified. The established approach can be utilized for routine, quality control analysis of cordycepin, as it is easy to use, sensitive, accurate, precise, repeatable, and most importantly, cost-effective.