The Comparative Cytotoxic Activities of Lutein and Cisplatin in Combination with Liposomes in Relation to Breast Tumor Cells Exposed to Radiotherapy

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-03-07 DOI:10.1134/S0006350923050135
R. A. Lafta, M. W. Shafaa, W. M. Darwish, M. S. El-Nagdy
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Abstract

The interactions of the antitumor substance cisplatin and the antioxidant substance lutein with liposomes, which are considered as model membranes, have been characterized. The morphology of all liposomes was almost spherical; in the absence of the studied substances, liposomes were more evenly distributed by size and less prone to aggregation. The average size of unloaded liposomes was 617.90 ± 75.64 nm; after inclusion of cisplatin, lutein and their combination, it was 425.60 ± 64.74 nm, 877.85 ± 93.90 nm, and 189.91 ± 136.84 nm, respectively. The inclusion of cisplatin or lutein into liposomal membranes caused an increase in the zeta potential. In the presence of cisplatin in combination with lutein, the zeta potential reached the lowest values. The inclusion of cisplatin into liposomes led to a shift of the main melting point peak towards higher temperature compared to unloaded liposomes, which indicated a conformational violation of the structure of phospholipids. The addition of lutein caused the disappearance of the main endothermic peak characteristic of pure liposomes. A study by Fourier-transform infrared spectroscopy confirmed the interaction of lutein and cisplatin with functional groups of liposomes. In the absence of gamma irradiation, the IC50 of lutein in the cytotoxicity test with MCF-7 cells was 10.62 μg/mL; the IC50 of cisplatin was 41.02 μg/mL. The IC50 of lutein and cisplatin in nanoliposomes was 65.84 and 34.29 μg/mL, respectively. In combination therapy with a dose of gamma radiation of 5 and 10 Gy, the IC50 of lutein decreased from 17.0 to 9.5 μg/mL. The IC50 of cisplatin changed from 51.00 to 43.09 μg/mL. This study showed that the cytotoxic effect of the standard form of lutein was stronger than that of lutein in nanoliposomes. According to this, a new therapy protocol can be proposed in which cisplatin should be replaced with lutein to increase the effectiveness of therapy against MCF-7 tumor cells.

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叶黄素和顺铂与脂质体结合对接受放疗的乳腺肿瘤细胞的细胞毒活性比较
摘要 研究人员对抗肿瘤物质顺铂和抗氧化物质叶黄素与脂质体(被视为模型膜)的相互作用进行了表征。所有脂质体的形态几乎都是球形的;在没有所研究物质的情况下,脂质体的大小分布更均匀,不易聚集。无负载脂质体的平均粒径为 617.90 ± 75.64 nm;加入顺铂、叶黄素和它们的复合物后,平均粒径分别为 425.60 ± 64.74 nm、877.85 ± 93.90 nm 和 189.91 ± 136.84 nm。在脂质体膜中加入顺铂或叶黄素会导致 zeta 电位增加。在顺铂与叶黄素结合的情况下,zeta 电位达到最低值。与未加载的脂质体相比,在脂质体中加入顺铂会导致主熔点峰向更高温度移动,这表明磷脂的结构发生了构象变化。叶黄素的加入导致纯脂质体特有的主要内热峰消失。傅立叶变换红外光谱研究证实了叶黄素和顺铂与脂质体功能基团的相互作用。在没有伽马射线照射的情况下,叶黄素对 MCF-7 细胞的细胞毒性测试的 IC50 为 10.62 μg/mL;顺铂的 IC50 为 41.02 μg/mL。纳米脂质体中叶黄素和顺铂的 IC50 分别为 65.84 μg/mL 和 34.29 μg/mL。在与剂量为 5 和 10 Gy 的伽马射线联合治疗时,叶黄素的 IC50 从 17.0 μg/mL 降至 9.5 μg/mL。顺铂的 IC50 从 51.00 μg/mL 降至 43.09 μg/mL。这项研究表明,标准叶黄素的细胞毒性作用强于纳米脂质体中的叶黄素。据此,可以提出一种新的治疗方案,用叶黄素替代顺铂,以提高对MCF-7肿瘤细胞的治疗效果。
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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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