R. A. Lafta, M. W. Shafaa, W. M. Darwish, M. S. El-Nagdy
{"title":"The Comparative Cytotoxic Activities of Lutein and Cisplatin in Combination with Liposomes in Relation to Breast Tumor Cells Exposed to Radiotherapy","authors":"R. A. Lafta, M. W. Shafaa, W. M. Darwish, M. S. El-Nagdy","doi":"10.1134/S0006350923050135","DOIUrl":null,"url":null,"abstract":"<p>The interactions of the antitumor substance cisplatin and the antioxidant substance lutein with liposomes, which are considered as model membranes, have been characterized. The morphology of all liposomes was almost spherical; in the absence of the studied substances, liposomes were more evenly distributed by size and less prone to aggregation. The average size of unloaded liposomes was 617.90 ± 75.64 nm; after inclusion of cisplatin, lutein and their combination, it was 425.60 ± 64.74 nm, 877.85 ± 93.90 nm, and 189.91 ± 136.84 nm, respectively. The inclusion of cisplatin or lutein into liposomal membranes caused an increase in the zeta potential. In the presence of cisplatin in combination with lutein, the zeta potential reached the lowest values. The inclusion of cisplatin into liposomes led to a shift of the main melting point peak towards higher temperature compared to unloaded liposomes, which indicated a conformational violation of the structure of phospholipids. The addition of lutein caused the disappearance of the main endothermic peak characteristic of pure liposomes. A study by Fourier-transform infrared spectroscopy confirmed the interaction of lutein and cisplatin with functional groups of liposomes. In the absence of gamma irradiation, the <i>IC</i><sub>50</sub> of lutein in the cytotoxicity test with MCF-7 cells was 10.62 μg/mL; the <i>IC</i><sub>50</sub> of cisplatin was 41.02 μg/mL. The <i>IC</i><sub>50</sub> of lutein and cisplatin in nanoliposomes was 65.84 and 34.29 μg/mL, respectively. In combination therapy with a dose of gamma radiation of 5 and 10 Gy, the <i>IC</i><sub>50</sub> of lutein decreased from 17.0 to 9.5 μg/mL. The <i>IC</i><sub>50</sub> of cisplatin changed from 51.00 to 43.09 μg/mL. This study showed that the cytotoxic effect of the standard form of lutein was stronger than that of lutein in nanoliposomes. According to this, a new therapy protocol can be proposed in which cisplatin should be replaced with lutein to increase the effectiveness of therapy against MCF-7 tumor cells.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 5","pages":"808 - 822"},"PeriodicalIF":4.0330,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350923050135","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
The interactions of the antitumor substance cisplatin and the antioxidant substance lutein with liposomes, which are considered as model membranes, have been characterized. The morphology of all liposomes was almost spherical; in the absence of the studied substances, liposomes were more evenly distributed by size and less prone to aggregation. The average size of unloaded liposomes was 617.90 ± 75.64 nm; after inclusion of cisplatin, lutein and their combination, it was 425.60 ± 64.74 nm, 877.85 ± 93.90 nm, and 189.91 ± 136.84 nm, respectively. The inclusion of cisplatin or lutein into liposomal membranes caused an increase in the zeta potential. In the presence of cisplatin in combination with lutein, the zeta potential reached the lowest values. The inclusion of cisplatin into liposomes led to a shift of the main melting point peak towards higher temperature compared to unloaded liposomes, which indicated a conformational violation of the structure of phospholipids. The addition of lutein caused the disappearance of the main endothermic peak characteristic of pure liposomes. A study by Fourier-transform infrared spectroscopy confirmed the interaction of lutein and cisplatin with functional groups of liposomes. In the absence of gamma irradiation, the IC50 of lutein in the cytotoxicity test with MCF-7 cells was 10.62 μg/mL; the IC50 of cisplatin was 41.02 μg/mL. The IC50 of lutein and cisplatin in nanoliposomes was 65.84 and 34.29 μg/mL, respectively. In combination therapy with a dose of gamma radiation of 5 and 10 Gy, the IC50 of lutein decreased from 17.0 to 9.5 μg/mL. The IC50 of cisplatin changed from 51.00 to 43.09 μg/mL. This study showed that the cytotoxic effect of the standard form of lutein was stronger than that of lutein in nanoliposomes. According to this, a new therapy protocol can be proposed in which cisplatin should be replaced with lutein to increase the effectiveness of therapy against MCF-7 tumor cells.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍:
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.