The Composition and Mitogenic Activity of Polysaccharide from Solanum tuberosum L.

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-03-07 DOI:10.1134/S0006350923050093
E. A. Generalov, L. V. Yakovenko
{"title":"The Composition and Mitogenic Activity of Polysaccharide from Solanum tuberosum L.","authors":"E. A. Generalov,&nbsp;L. V. Yakovenko","doi":"10.1134/S0006350923050093","DOIUrl":null,"url":null,"abstract":"<p>The STP polysaccharide was isolated from an aqueous extract of <i>Solanum tuberosum</i> L. and purified by ion exchange chromatography and gel filtration. Its molecular mass was determined by gel-penetrating chromatography and high-performance liquid chromatography, and its monosaccharide composition was analyzed by high-performance liquid chromatography and gas chromatography using a flame ionization detector and a capillary column. It was shown that STP polysaccharide consists of galactose and arabinose at 37.5% and 23.5%, respectively, as well as uronic acids (9.7%), glucose monosaccharide residues (15%), and proteins (at least 9%). The molecular mass of STP is 70 kDa. The method of IR-Fourier spectroscopy was used for the structural analysis of STP. The mitogenic activity of the extracted polysaccharide was comparable to the activity of lipopolysaccharide.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 5","pages":"698 - 704"},"PeriodicalIF":4.0330,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350923050093","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

Abstract

The STP polysaccharide was isolated from an aqueous extract of Solanum tuberosum L. and purified by ion exchange chromatography and gel filtration. Its molecular mass was determined by gel-penetrating chromatography and high-performance liquid chromatography, and its monosaccharide composition was analyzed by high-performance liquid chromatography and gas chromatography using a flame ionization detector and a capillary column. It was shown that STP polysaccharide consists of galactose and arabinose at 37.5% and 23.5%, respectively, as well as uronic acids (9.7%), glucose monosaccharide residues (15%), and proteins (at least 9%). The molecular mass of STP is 70 kDa. The method of IR-Fourier spectroscopy was used for the structural analysis of STP. The mitogenic activity of the extracted polysaccharide was comparable to the activity of lipopolysaccharide.

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
茄科植物多糖的成分和有丝分裂活性
摘要 从茄科植物Solanum tuberosum L.的水提取物中分离出STP多糖,并通过离子交换色谱和凝胶过滤进行纯化。用凝胶穿透色谱法和高效液相色谱法测定了其分子质量,并用高效液相色谱法和气相色谱法(火焰离子化检测器和毛细管柱)分析了其单糖组成。结果表明,STP 多糖由半乳糖和阿拉伯糖组成,分别占 37.5%和 23.5%,此外还有尿酸(9.7%)、葡萄糖单糖残基(15%)和蛋白质(至少 9%)。STP 的分子质量为 70 kDa。红外-傅里叶光谱法用于分析 STP 的结构。提取多糖的有丝分裂活性与脂多糖的活性相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
期刊最新文献
Reductive Nitrosylation of Hemoglobin and Myoglobin and its Antioxidant Effect Supercomputer Simulation of Intramolecular Oscillations of Glycine, Diphenylalanine, and Tryptophan in an Electric Field of the Terahertz and Infrared Ranges On the Role of Priming in the Development of Modern Rehabilitation Technologies The Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E An X-Ray Diffraction Study of Lipid Films with ICHPHAN
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1