Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Abstract

This study aimed to establish two methods, namely, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic test strips, for detecting prometryn residues in various agricultural samples, such as corn, pumpkin, lotus root, rice, and brown rice. Monoclonal antibodies specific for prometryn were utilized in both methods. The ic-ELISA method demonstrated a maximum half-inhibition concentration (IC₅₀) of 2.4 ng/mL and a lower limit of detection of 0.5 ng/mL. When used in conjunction with a card reader, the colloidal gold immunochromatographic test strip exhibited an IC₅₀ of 18.6 ng/mL for prometryn detection. The recovery rates of both methods were found to be consistent with those of liquid chromatography tandem mass spectrometry. This consistency indicated the suitability of both methods for detecting prometryn in five different agricultural samples, suggesting that this is a convenient and practical approach for screening prometryn in agricultural products.