Growth and Immunity Performance of Nile Tilapia (Oreochromis niloticus) Challenged by Toxicity of Bio-Insecticide with Active Ingredients Eugenol and Azadirachtin

Abstract

This study aims to determine the maximum concentration and the long-term effects after exposure to a bio-insecticide with active ingredients eugenol and azadirachtin on the survival rate, immunity, and growth of Nile tilapia. The method used in this study was experimental, using a completely randomized design (CRD) with six treatments and three replications. Fishes were exposed to eugenol and azadirachtin at concentrations 10, 20, 30, 40, and 50% of LC50 value for 14 days, followed by 14 days of maintenance to see the effect on growth. The results showed that 66 mg.L-1 treatment was a concentration that did not interfere with the survival rate of Nile tilapia, which was 86.7%. The number of leukocytes increased on the third day by the highest increase in 66 mg.L-1 treatment at 12.01 × 104 cells.mm-3. Meanwhile, erythrocytes decreased, with the highest decrease in 66 mg.L-1 treatment at 1.13 × 106 cells.mm-3. The average growth rate in fish slowed down with increasing concentrations of exposure, with the lowest average growth in length and absolute weight in the 66 mg.L-1 treatment was 0.57 cm and 1.68 g.