Treatment Promotion of Osteoporotic Fractures by microRNA-320 Nanocapsules Through Stimulating Bone Marrow Mesenchymal Stem Cells

Abstract

We aimed to explore the mechanism underlying microRNA-320 (miR-320)’s role in osteoporotic fractures. miR-320 nanoparticles were prepared and their characterization was detected by Zetasizer Nano and triethylamine (TEA). miR-320 nanoparticles were interacted with bone marrow mesenchymal stem cells (BMSCs). Then we conducted MTT to assess cytotoxicity in BMSCs and determined genes expression. A mouse fracture model was established and treated with miR-320 nanoparticles or pore nanoparticles. The release of miR-320 and the bone repair at the fracture site were detected. Treatment of Ceramic matrix composites (CMCS) (miR-320) sensitive to Matrix metalloproteinase (MMP) released miR-320 to bone defect, which promoted the transcription of osteogenic genes and stimulated the osteogenesis. Finally, treatment of miR-320 nanoparticles facilitated bone repair of mouse osteoporotic defect. MMP-sensitive nanocapsules loaded with miR-320 can promote osteogenic potential and stimulate fracture repair, providing insight into novel treatment against osteoporotic fracture.