The Effect of Silk Fibroin/Poly(Lactic-Co-Glycolic Acid) Nanofiber Coating on Porous Calcium Phosphate-Based Composites for Osteoporosis Application

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-03-01 DOI:10.1166/jbn.2024.3785

Qiao Ren, Qinggui Li, Ligang Qian

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Abstract

This study observed whether the silk fibroin/poly lactic-co-glycolic acid (SF/PLGA) nanofiber membrane three-dimensionally coated with porous calcium phosphate-based composite can promote the proliferation of osteoblasts, thereby improving osteoporosis. The SF/PLGA nanofiber membrane was used to three-dimensionally coated with the porous calcium phosphate-based composite to analyze proliferation of osteoblasts, hFOB1.19 expressions by Western blot, activity change of hFOB1.19 by CCK-8 assay. The morphology was observed with an electron microscope. Alizarin Red staining showed that the SF/PLGA nanofiber membrane three-dimensionally coated with porous calcium phosphatebased composite promoted osteogenic differentiation and upregulated the expression of osteogenic related genes. It inhibited adipogenic differentiation and the expression of adipogenic genes. SF/PLGA composite material promoted the viability of osteoblasts hFOB1.19, upregulatedGLUT3, downregulated the expression of various apoptosis-related proteins in hFOB1.19. SF/PLGA composite material can reduce the apoptosis efficiency of osteoblasts, promote the proliferation of osteoblasts, and inhibit apoptosis, thereby improving osteoporosis.

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蚕丝纤维素/聚（乳酸-共羟基乙酸）纳米纤维涂层对骨质疏松症用多孔磷酸钙基复合材料的影响

本研究观察了丝纤维素/聚乳酸-乙醇酸（SF/PLGA）纳米纤维膜三维包覆多孔磷酸钙基复合材料能否促进成骨细胞增殖，从而改善骨质疏松症。利用SF/PLGA纳米纤维膜三维包覆多孔磷酸钙基复合材料，分析了成骨细胞的增殖、Western blot检测hFOB1.19的表达、CCK-8检测hFOB1.19的活性变化。用电子显微镜观察其形态。茜素红染色表明，SF/PLGA 纳米纤维膜三维包覆多孔磷酸钙基复合材料可促进成骨分化，并上调成骨相关基因的表达。它抑制了成脂分化和成脂基因的表达。SF/PLGA 复合材料能促进成骨细胞 hFOB1.19 的活力，上调 GLUT3，下调 hFOB1.19 中各种凋亡相关蛋白的表达。SF/PLGA复合材料能降低成骨细胞的凋亡效率，促进成骨细胞增殖，抑制细胞凋亡，从而改善骨质疏松症。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.