Characterization of tumor microbiome and associations with prognosis in intrahepatic cholangiocarcinoma.

IF 6.9 2区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Journal of Gastroenterology Pub Date : 2024-05-01 Epub Date: 2024-03-10 DOI:10.1007/s00535-024-02090-2

Hao-Yang Xin, Ji-Xue Zou, Rong-Qi Sun, Zhi-Qiang Hu, Zhuo Chen, Chu-Bin Luo, Zheng-Jun Zhou, Peng-Cheng Wang, Jia Li, Song-Yang Yu, Kai-Xuan Liu, Jia Fan, Jian Zhou, Shao-Lai Zhou

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Abstract

Background: The tumor microbiome has been characterized in several malignancies; however, no previous studies have investigated its role in intrahepatic cholangiocarcinoma (ICC). Hence, we explored the tumor microbiome and its association with prognosis in ICC.

Methods: One hundred and twenty-one ICC tumor samples and 89 adjacent normal tissues were profiled by 16S rRNA sequencing. Microbial differences between tumor and adjacent nontumoral liver tissues were assessed. Tumor microbial composition was then evaluated to detect its association with prognosis. Finally, a risk score calculated by the tumor microbiota was accessed by the least absolute shrinkage and selector operator method (Lasso) to predict prognosis of ICC.

Results: The tumor microbiome displayed a greater diversity than that in adjacent nontumoral liver tissues. Tumor samples were characterized by a higher abundance of Firmicutes, Actinobacteria, Bacteroidetes, and Acidobacteriota. Higher tumor microbial α diversity was associated with lymph node metastasis and predicted shortened overall survival (OS) and recurrence-free survival (RFS). A total of 11 bacteria were selected to generate the risk score by Lasso. This score showed potential in predicting OS, and was an independent risk factor for OS.

Conclusion: In conclusion, our study characterized the tumor microbiome and revealed its role in predicting prognosis in ICC.

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肝内胆管癌的肿瘤微生物组特征及其与预后的关系

背景：肿瘤微生物组在几种恶性肿瘤中都有表征，但以前没有研究调查过其在肝内胆管癌（ICC）中的作用。因此，我们探讨了肿瘤微生物组及其与 ICC 预后的关系：方法：对121份ICC肿瘤样本和89份邻近正常组织进行了16S rRNA测序。评估了肿瘤和邻近非肿瘤肝组织之间的微生物差异。然后评估肿瘤微生物组成，检测其与预后的关系。最后，通过最小绝对缩减和选择算子法（Lasso）获得了由肿瘤微生物群计算出的风险评分，以预测ICC的预后：结果：与邻近的非肿瘤性肝组织相比，肿瘤微生物组显示出更高的多样性。肿瘤样本的特点是富含更多的固缩菌、放线菌、类杆菌和酸性杆菌。较高的肿瘤微生物α多样性与淋巴结转移有关，并预示着总生存期（OS）和无复发生存期（RFS）的缩短。通过 Lasso 方法，共筛选出 11 种细菌生成风险评分。该评分显示了预测OS的潜力，并且是OS的独立风险因素：总之，我们的研究描述了肿瘤微生物组的特征，并揭示了其在预测 ICC 预后中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Gastroenterology 医学-胃肠肝病学

CiteScore

12.20

自引率

1.60%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Gastroenterology, which is the official publication of the Japanese Society of Gastroenterology, publishes Original Articles (Alimentary Tract/Liver, Pancreas, and Biliary Tract), Review Articles, Letters to the Editors and other articles on all aspects of the field of gastroenterology. Significant contributions relating to basic research, theory, and practice are welcomed. These publications are designed to disseminate knowledge in this field to a worldwide audience, and accordingly, its editorial board has an international membership.