Roots of Rosa majalis Herrm. as a source of antioxidants and anti-influenza agents

IF 2.1 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Rendiconti Lincei-Scienze Fisiche E Naturali Pub Date : 2024-03-11 DOI:10.1007/s12210-024-01230-3

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Abstract

Rosa majalis Herrm. (Rosaceae) is a shrub widespread throughout Eurasia. In West Siberia, it is cultivated as an ornamental plant and raw material for the manufacture of fruit multivitamin concentrates. Meanwhile, metabolites and biological activities of its roots are poorly studied. The aim of this investigation was to evaluate the potential of ethyl acetate (EtOAc), butanol (ButOH), aqueous ethanol (EtOH), and aqueous (H₂O) extracts of R. majalis roots as sources of antioxidant and anti-influenza compounds. Spectrophotometry and HPLC were used for metabolite quantification. Antioxidant activities were determined via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The antiviral activities were assessed as half-maximal inhibitory concentrations (IC₅₀). The EtOAc extract manifested the highest concentrations of phenolic compounds (53.79 mg g⁻¹ of dry extract) and saponins (48.92 mg g⁻¹). Total gallic acid was the main phenolic compound in all extracts (24.98–38.68 mg g⁻¹). Catechin, free ellagic acid, and total ellagic acid had the highest levels in the EtOAc, H₂O, and EtOH extracts, respectively (30.83, 22.38, and 34.95 mg g⁻¹). The EtOAc extract showed the highest free-radical scavenging activity (2.31 trolox equivalents) and anti-influenza effects (IC₅₀ 61.8 and 50.3 µg mL⁻¹) against A/Aichi/2/68 (H3N2) and A/Chicken/05/2005 (H5N1), respectively. Our findings indicate good potential of these extracts for drug design against free-radical damage and influenza virus.

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作为抗氧化剂和抗流感剂来源的蔷薇根

摘要 Rosa majalis Herrm.（蔷薇科）是一种遍布欧亚大陆的灌木。在西西伯利亚，它被作为观赏植物和制造水果多种维生素浓缩物的原料进行栽培。与此同时，人们对其根部的代谢物和生物活性研究甚少。本研究旨在评估大叶女贞根的乙酸乙酯（EtOAc）、丁醇（ButOH）、乙醇水溶液（EtOH）和水溶液（H2O）提取物作为抗氧化和抗流感化合物来源的潜力。采用分光光度法和高效液相色谱法对代谢物进行定量。抗氧化活性通过 2,2-二苯基-1-苦基肼（DPPH）测定法确定。抗病毒活性以半最大抑制浓度（IC50）进行评估。乙酸乙酯提取物中的酚类化合物（53.79 毫克/克-1 干提取物）和皂苷（48.92 毫克/克-1）含量最高。总没食子酸是所有提取物中的主要酚类化合物（24.98-38.68 毫克/克-1）。儿茶素、游离鞣花酸和总鞣花酸在 EtOAc、H2O 和 EtOH 提取物中含量最高，分别为 30.83、22.38 和 34.95 mg g-1。EtOAc 提取物对 A/Aichi/2/68（H3N2）和 A/Chicken/05/2005（H5N1）分别显示出最高的自由基清除活性（2.31 托罗克斯当量）和抗流感效果（IC50 61.8 和 50.3 µg mL-1）。我们的研究结果表明，这些提取物在抗自由基损伤和流感病毒的药物设计方面具有良好的潜力。

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来源期刊

Rendiconti Lincei-Scienze Fisiche E Naturali MULTIDISCIPLINARY SCIENCES-

CiteScore

4.10

自引率

10.00%

发文量

审稿时长

>12 weeks

期刊介绍： Rendiconti is the interdisciplinary scientific journal of the Accademia dei Lincei, the Italian National Academy, situated in Rome, which publishes original articles in the fi elds of geosciences, envi ronmental sciences, and biological and biomedi cal sciences. Particular interest is accorded to papers dealing with modern trends in the natural sciences, with interdisciplinary relationships and with the roots and historical development of these disciplines.