Methods to Study the Role of Mechanical Signals in the Induction of Cancer Stem Cells.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3730-2_13
Alessandro Gandin, Paolo Contessotto, Tito Panciera
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Abstract

Microenvironmental mechanical signals are fundamental regulators of cell behavior both in physiological and in pathological context, particularly in the induction and maintenance of tumorigenic properties. It is thus of utmost importance to experimentally recreate conditions that mimic the physical attributes of real tissues to study their impact on cell behavior and in particular on the induction of cancer stem cell (CSC) properties. Here we present protocols to investigate the role of mechanical stiffness on reprogramming of primary mammary gland cells into CSCs, including the synthesis of hydrogel substrates of the desired stiffness, the isolation and culture of primary differentiated normal cells derived from the human mammary gland, and the assessment of their CSC attributes after oncogene-mediated transformation.

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研究机械信号在诱导癌症干细胞中的作用的方法。
微环境机械信号是细胞生理和病理行为的基本调节器,尤其是在诱导和维持致瘤特性方面。因此,最重要的是在实验中模拟真实组织的物理属性,研究它们对细胞行为的影响,特别是对诱导癌症干细胞(CSC)特性的影响。在这里,我们介绍了研究机械硬度对原代乳腺细胞重编程为 CSC 的作用的方案,包括合成所需硬度的水凝胶基底、分离和培养来自人类乳腺的原代分化正常细胞,以及评估它们在癌基因介导的转化后的 CSC 属性。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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