Relative survival and detoxification enzyme activity in Dysdercus koenigii (Hemiptera: Pyrrhocoridae) exposed to β-cyfluthrin alone and its nanometric emulsion

IF 1.5 3区农林科学 Q2 AGRONOMY Phytoparasitica Pub Date : 2024-03-13 DOI:10.1007/s12600-024-01156-4

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引用次数: 0

Abstract

Dysdercus koenigii, a global cotton pest, inflicts significant crop losses via sucking the moisture and oil contents from leaves and seeds. The growing environmental hazards with conventional pesticides have led to the exploration of nanotechnology to formulate alternative products and nanoemulsions as suitable insecticide delivery vehicles for target pests. We formulated nanoemulsions using β-cyfluthrin (a pyrethroid) and DMSO (Dimethyl sulfoxide) in 1:2, 1:4, 1:6, 1:8 and 1:10 proportions. The nanoemulsions (NE) were characterized morphologically and biophysically (size, pH, viscosity, PDI and zeta potential) by Transmission Electron Microscopy, Dynamic Light Scattering and Zeta Potential Analyzer. Each NE was transparent, stable, had 11.4–174.1 nm droplet size and, optimal PDI and zeta potential. The spherical and monodispersed 1:2 β-cyfluthrin + DMSO NE was found as the most optimal formulation inducing 100% mortality in D. koenigii fifth instars after 24 h; the NE (LD₅₀ = 3.536 mg/L) displayed 44% higher efficacy than the β-cyfluthrin alone (LD₅₀ = 5.059 mg/L). The LD₅₀ dose of the 1:2 NE enhanced the activity of α-esterase, β-esterase, glutathione-S-transferase, and acetylcholinesterase in nymphs in comparison with β-cyfluthrin alone. The β-cyfluthrin nanoemulsion can act as a potential tool to manage D. koenigii after thorough field assessment and evaluation of the impact on non-target organisms.

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暴露于单独的β-氟氯氰菊酯及其纳米乳剂的鹅膏蕈蚊（半翅目：鹅膏蕈科）的相对存活率和解毒酶活性

摘要柯尼希蝇（Dysdercus koenigii）是一种全球性棉花害虫，通过吸食叶片和种子中的水分和油分，对作物造成重大损失。由于传统杀虫剂对环境的危害日益严重，人们开始探索利用纳米技术配制替代产品，并将纳米乳剂作为杀虫剂的运载工具，用于防治目标害虫。我们使用 β-氟氯氰菊酯（一种拟除虫菊酯）和 DMSO（二甲基亚砜）以 1:2、1:4、1:6、1:8 和 1:10 的比例配制了纳米乳剂。利用透射电子显微镜、动态光散射和 Zeta 电位分析仪对纳米乳液（NE）进行了形态学和生物物理学表征（粒度、pH 值、粘度、PDI 和 Zeta 电位）。每种 NE 均透明、稳定，液滴大小为 11.4-174.1 nm，PDI 和 Zeta 电位均为最佳值。研究发现，球形单分散 1:2 β-氟氯氰菊酯 + DMSO NE 是最理想的制剂，可在 24 小时后诱导柯尼希幼虫第五龄幼虫 100%死亡；该 NE 的药效（LD50 = 3.536 mg/L）比单独使用 β-氟氯氰菊酯的药效（LD50 = 5.059 mg/L）高 44%。与单独使用β-氟氯氰菊酯相比，1:2 NE 的 LD50 剂量可提高若虫体内α-酯酶、β-酯酶、谷胱甘肽-S-转移酶和乙酰胆碱酯酶的活性。在对非目标生物的影响进行彻底的实地评估和评价后，β-氟氯氰菊酯纳米乳剂可作为一种潜在的工具来管理矢车菊。

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来源期刊

Phytoparasitica 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytoparasitica is an international journal on Plant Protection, that publishes original research contributions on the biological, chemical and molecular aspects of Entomology, Plant Pathology, Virology, Nematology, and Weed Sciences, which strives to improve scientific knowledge and technology for IPM, in forest and agroecosystems. Phytoparasitica emphasizes new insights into plant disease and pest etiology, epidemiology, host-parasite/pest biochemistry and cell biology, ecology and population biology, host genetics and resistance, disease vector biology, plant stress and biotic disorders, postharvest pathology and mycotoxins. Research can cover aspects related to the nature of plant diseases, pests and weeds, the causal agents, their spread, the losses they cause, crop loss assessment, and novel tactics and approaches for their management.