miR-107 and miR-126 and Risk of Breast Cancer: A Case-Control Study.

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY Genetic testing and molecular biomarkers Pub Date : 2024-04-01 Epub Date: 2024-03-15 DOI:10.1089/gtmb.2023.0606
Priyanka Gupta, Vasudha Sambyal, Jagmohan Singh Bali, Kamlesh Guleria, Manjit Singh Uppal, Meena Sudan
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Abstract

Background: Micro RNAs are new diagnostic markers and therapeutic targets in breast cancer research. miR-107 and miR-126 have been reported to be linked with the pathogenesis of breast cancer. The present study investigates the levels of expression of miR-107 and miR-126 in patients with breast cancer to find their correlation with the risk of breast cancer in Amritsar, Punjab, Northwest India. Material and Methods: In total, 200 subjects, 100 patients with breast cancer and 100 controls, were enrolled to screen the expression of miR-107 and miR-126 using quantitative reverse transcription polymerase chain reaction (RT-PCR) technique. The Livak method (2-ΔΔCt) was used to calculate the fold change of the expression of micro RNAs. Student t-test was used to calculate the significant change in the expression of miRNAs in patients as compared with controls. Spearman rank correlation coefficient and ROC were conducted. The value of p < 0.05 was considered to indicate a statistically significant difference. Results: miR-107 was downregulated in patients with breast cancer as compared with controls (fold change = 0.467; p = 0.114) but not statistically significant. The expression of miR-126 was found to be 5.37 times elevated in patients with breast cancer, specifically in stage I and stage III patients (p = 0.009), compared with controls, which may indicate its oncogenic activity. The ROC analyses revealed that miR-126 could be a potential diagnostic marker. In conclusion oncogenic behavior of miR-126 is suggestive of its role in pathogenesis in patients with breast cancer.

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miR-107 和 miR-126 与乳腺癌风险:病例对照研究
背景:据报道,miR-107 和 miR-126 与乳腺癌的发病机制有关。本研究调查了印度西北部旁遮普省阿姆利则市乳腺癌患者体内 miR-107 和 miR-126 的表达水平,以发现它们与乳腺癌风险的相关性。材料和方法:共招募了 200 名受试者(100 名乳腺癌患者和 100 名对照组),使用定量反转录聚合酶链反应(RT-PCR)技术筛查 miR-107 和 miR-126 的表达。采用 Livak 法(2-ΔΔCt)计算微 RNA 表达的折叠变化。采用学生 t 检验计算患者与对照组相比 miRNA 表达的显著变化。进行斯皮尔曼秩相关系数和 ROC 检验。p < 0.05 的值被认为表示差异有统计学意义。结果:与对照组相比,乳腺癌患者的 miR-107 表达下调(折叠变化 = 0.467; p = 0.114),但无统计学意义。与对照组相比,miR-126 在乳腺癌患者(尤其是 I 期和 III 期患者)中的表达量升高了 5.37 倍(p = 0.009),这可能表明其具有致癌活性。ROC 分析显示,miR-126 可能是一种潜在的诊断标志物。总之,miR-126 的致癌行为表明它在乳腺癌患者的发病机制中发挥作用。
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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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