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{"title":"Thiosulfinate Tolerance Gene Clusters Are Common Features of <i>Burkholderia</i> Onion Pathogens.","authors":"Sujan Paudel, Mei Zhao, Shaun P Stice, Bhabesh Dutta, Brian H Kvitko","doi":"10.1094/MPMI-01-24-0005-R","DOIUrl":null,"url":null,"abstract":"<p><p><i>Burkholderia gladioli</i> pv. <i>alliicola</i>, <i>B. cepacia</i>, and <i>B. orbicola</i> are common bacterial pathogens of onion. Onions produce organosulfur thiosulfinate defensive compounds after cellular decompartmentalization. Using whole-genome sequencing and in silico analysis, we identified putative thiosulfinate tolerance gene (TTG) clusters in multiple onion-associated <i>Burkholderia</i> species similar to those characterized in other <i>Allium</i>-associated bacterial endophytes and pathogens. Sequence analysis revealed the presence of three <i>Burkholderia</i> TTG cluster types, with both Type A and Type B being broadly distributed in <i>B. gladioli</i>, <i>B. cepacia</i>, and <i>B. orbicola</i> in both the chromosome and plasmids. Based on isolate natural variation and generation of isogenic strains, we determined the in vitro and in vivo contribution of TTG clusters in <i>B. gladioli</i>, <i>B. cepacia</i>, and <i>B. orbicola</i>. The <i>Burkholderia</i> TTG clusters contributed to enhanced allicin tolerance and improved growth in filtered onion extracts by all three species. TTG clusters also made clear contributions to <i>B. gladioli</i> foliar necrosis symptoms and bacterial populations. Surprisingly, the TTG cluster did not contribute to bacterial populations in onion bulb scales by these three species. Based on our findings, we hypothesize onion-associated <i>Burkholderia</i> may evade or inhibit the production of thiosulfinates in onion bulb tissues. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</p>","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"507-519"},"PeriodicalIF":3.2000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Plant-microbe Interactions","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1094/MPMI-01-24-0005-R","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/18 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
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Abstract
Burkholderia gladioli pv. alliicola , B. cepacia , and B. orbicola are common bacterial pathogens of onion. Onions produce organosulfur thiosulfinate defensive compounds after cellular decompartmentalization. Using whole-genome sequencing and in silico analysis, we identified putative thiosulfinate tolerance gene (TTG) clusters in multiple onion-associated Burkholderia species similar to those characterized in other Allium -associated bacterial endophytes and pathogens. Sequence analysis revealed the presence of three Burkholderia TTG cluster types, with both Type A and Type B being broadly distributed in B. gladioli , B. cepacia , and B. orbicola in both the chromosome and plasmids. Based on isolate natural variation and generation of isogenic strains, we determined the in vitro and in vivo contribution of TTG clusters in B. gladioli , B. cepacia , and B. orbicola . The Burkholderia TTG clusters contributed to enhanced allicin tolerance and improved growth in filtered onion extracts by all three species. TTG clusters also made clear contributions to B. gladioli foliar necrosis symptoms and bacterial populations. Surprisingly, the TTG cluster did not contribute to bacterial populations in onion bulb scales by these three species. Based on our findings, we hypothesize onion-associated Burkholderia may evade or inhibit the production of thiosulfinates in onion bulb tissues. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
硫代硫酸耐受基因簇是洋葱伯克霍尔德氏菌病原体的共同特征。
Burkholderia gladioli pv. alliicola、B.cepacia 和 B. orbicola 是洋葱的常见细菌病原体。洋葱在细胞分解后会产生有机硫硫酸盐防御化合物。利用全基因组测序和硅分析,我们在多种洋葱相关伯克霍尔德氏菌中发现了假定的硫代硫酸盐耐受基因(TTG)群,这些基因群与其他薤相关细菌内生菌和病原体中的基因群相似。序列分析表明存在三种伯克霍尔德氏菌 TTG 簇类型,其中 A 型和 B 型广泛分布于 B. gladioli、B. cepacia 和 B. orbicola 的染色体和质粒中。根据分离物的自然变异和生成的同源菌株,我们确定了 TTG 簇在 B. gladioli、B. cepacia 和 B. orbicola 中的体外和体内贡献。伯克霍尔德氏菌 TTG 簇增强了大蒜素耐受性,并改善了所有三个物种在过滤洋葱提取物中的生长。TTG 菌群还对 B. gladioli 的叶片坏死症状和细菌数量做出了明显的贡献。令人惊讶的是,TTG 簇群对这三个物种在洋葱鳞茎中的细菌数量没有影响。根据我们的研究结果,我们推测洋葱相关伯克霍尔德氏菌可能会逃避或抑制洋葱鳞茎组织中硫代硫酸盐的产生。
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