MRI-guided photothermal/photodynamic immune activation combined with PD-1 inhibitor for the multimodal combination therapy of melanoma and metastases

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Regenerative Biomaterials Pub Date : 2024-03-13 DOI:10.1093/rb/rbae019
Changqiang Wu, Wei Chen, Shuang Yan, Jie Zhong, Liang Du, Chenwu Yang, Yu Pu, Yang Li, Jiafu Lin, Mei Zeng, Xiaoming Zhang
{"title":"MRI-guided photothermal/photodynamic immune activation combined with PD-1 inhibitor for the multimodal combination therapy of melanoma and metastases","authors":"Changqiang Wu, Wei Chen, Shuang Yan, Jie Zhong, Liang Du, Chenwu Yang, Yu Pu, Yang Li, Jiafu Lin, Mei Zeng, Xiaoming Zhang","doi":"10.1093/rb/rbae019","DOIUrl":null,"url":null,"abstract":"Non-invasive image-guided precise photothermal/photodynamic therapy (PTT/PDT) has been proven an effective local treatment modality, but incompetent against metastases. Hence the combination of local PTT/PDT and systemic immunotherapy would be a promising strategy for tumor eradication. Herein, a MRI-visualized PTT/PDT agent (SIDP NMs) was constructed, and the efficacy of its multimodal combination with PD-1 inhibitor in the treatment of melanoma and metastases was studied. Due to the hydrophobic encapsulation of indocyanine green (ICG) within the micellar core, SIDP NMs exhibited excellent photothermal/photodynamic properties and stability under 808 nm near-infrared laser (NIR). In vitro cell experiments showed that SIDP NMs had a good killing effect. After incubating with B16-F10 cells for 24 h and irradiating with 808 nm laser for 10 min, cell viability decreased significantly. Magnetic resonance imaging experiments in melanoma-bearing mice have shown that the dynamic distribution of SIDP NMs in tumor tissue could be monitored by T2WI and T2-MAP noninvasively due to the presence of superparamagnetic iron oxide nanocrystal (SPIO) in SIDP NMs. When the 808 nm laser was irradiated at the maximum focusing time point shown by MRI, the temperature of the tumor area rapidly increased from 32 °C to 60.7 °C in five minutes. In mouse melanoma ablation and distant tumor immunotherapy studies, SIDP NMs provided excellent MRI-guided photothermal/photodynamic therapy results and, when combined with PD-1 inhibitor, have great potential to cure primary tumors and eradicate metastases.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regenerative Biomaterials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/rb/rbae019","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

Non-invasive image-guided precise photothermal/photodynamic therapy (PTT/PDT) has been proven an effective local treatment modality, but incompetent against metastases. Hence the combination of local PTT/PDT and systemic immunotherapy would be a promising strategy for tumor eradication. Herein, a MRI-visualized PTT/PDT agent (SIDP NMs) was constructed, and the efficacy of its multimodal combination with PD-1 inhibitor in the treatment of melanoma and metastases was studied. Due to the hydrophobic encapsulation of indocyanine green (ICG) within the micellar core, SIDP NMs exhibited excellent photothermal/photodynamic properties and stability under 808 nm near-infrared laser (NIR). In vitro cell experiments showed that SIDP NMs had a good killing effect. After incubating with B16-F10 cells for 24 h and irradiating with 808 nm laser for 10 min, cell viability decreased significantly. Magnetic resonance imaging experiments in melanoma-bearing mice have shown that the dynamic distribution of SIDP NMs in tumor tissue could be monitored by T2WI and T2-MAP noninvasively due to the presence of superparamagnetic iron oxide nanocrystal (SPIO) in SIDP NMs. When the 808 nm laser was irradiated at the maximum focusing time point shown by MRI, the temperature of the tumor area rapidly increased from 32 °C to 60.7 °C in five minutes. In mouse melanoma ablation and distant tumor immunotherapy studies, SIDP NMs provided excellent MRI-guided photothermal/photodynamic therapy results and, when combined with PD-1 inhibitor, have great potential to cure primary tumors and eradicate metastases.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
核磁共振成像引导下的光热/光动力免疫激活结合 PD-1 抑制剂用于黑色素瘤和转移瘤的多模式联合治疗
事实证明,非侵入性图像引导精确光热/光动力疗法(PTT/PDT)是一种有效的局部治疗方式,但对转移瘤却无能为力。因此,将局部光热/光动力疗法与全身免疫疗法相结合将是一种很有前途的根除肿瘤的策略。本文构建了一种磁共振成像可视化 PTT/PDT 药剂(SIDP NMs),并研究了其与 PD-1 抑制剂多模式联合治疗黑色素瘤和转移瘤的疗效。由于吲哚菁绿(ICG)被疏水包裹在胶束内核中,SIDP NMs 在 808 纳米近红外激光(NIR)下表现出优异的光热/光动力特性和稳定性。体外细胞实验表明,SIDP NMs 具有良好的杀伤效果。与 B16-F10 细胞培养 24 小时并用 808 纳米激光照射 10 分钟后,细胞存活率显著下降。黑色素瘤小鼠的磁共振成像实验表明,由于 SIDP NMs 中含有超顺磁性氧化铁纳米晶(SPIO),因此可以通过 T2WI 和 T2-MAP 无创监测 SIDP NMs 在肿瘤组织中的动态分布。在核磁共振成像显示的最大聚焦时间点照射 808 纳米激光时,肿瘤区域的温度在五分钟内从 32 ℃ 迅速升高到 60.7 ℃。在小鼠黑色素瘤消融和远处肿瘤免疫治疗研究中,SIDP NMs 在核磁共振成像引导下提供了出色的光热/光动力治疗效果,与 PD-1 抑制剂结合使用时,在治疗原发性肿瘤和根除转移瘤方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
期刊最新文献
Correction to: Constructing a highly efficient multifunctional carbon quantum dot platform for the treatment of infectious wounds. Non-invasive fast assessment of hepatic injury through computed tomography imaging with renal-clearable Bi-DTPA dimeglumine. Hygroscopic bioactive light-cured composite promoting dentine bridge formation. Biological properties and characterization of several variations of a clinical human plasma-based skin substitute model and its manufacturing process. The effects of heat and hydrogen peroxide treatment on the osteoinductivity of demineralized cortical bone: a potential method for preparing tendon/ligament repair scaffolds.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1