Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Pub Date : 2024-03-17 DOI:10.1134/s0026893324700134

H. Zhou, S. J. Du, F. Gendi, H. Y. Li, J. Fu, C. Chang

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Abstract

The cadherin superfamily molecules, functioning as cell adhesion molecules, are recognized to play roles in both physiological and pathological processes. The cadherin-based adherent junction (CAJ) is believed to interact with presenilin-1 (PS-1), suggesting that disruptions in CAJ structures might contribute to neurodegeneration, potentially leading to Alzheimer’s Disease (AD). Yet, the specific expression patterns of cadherin superfamily mRNA remain somewhat ambiguous. This research utilized in situ hybridization (ISH) to examine the expression and localization of cadherin mRNA in AD mouse model brains. Long cRNA probes targeting cadherin revealed endogenous mRNA expression in brain sections. Interestingly, senile plaques in the AD mouse brain were also bound to these probes. This binding, however, did not exclusively denote cadherin mRNA, as ISH detected both antisense and sense cRNA probes. Our data suggest that although antisense cRNA probes effectively detected cadherin mRNA expression in AD brain cells, their association with senile plaques may not specifically indicate cadherin mRNA expression.

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钙粘连蛋白 cRNA 探针在阿尔茨海默病小鼠模型大脑中的应用

摘要粘连蛋白超家族分子作为细胞粘附分子，被认为在生理和病理过程中发挥作用。据信，以粘连蛋白为基础的粘连接头（CAJ）与预粘连蛋白-1（PS-1）相互作用，表明CAJ结构的破坏可能会导致神经退行性病变，从而可能导致阿尔茨海默病（AD）。然而，粘连蛋白超家族 mRNA 的具体表达模式仍然有些模糊。这项研究利用原位杂交（ISH）技术检测了AD模型小鼠大脑中粘连蛋白mRNA的表达和定位。靶向cadherin的长cRNA探针显示了脑切片中内源性mRNA的表达。有趣的是，AD小鼠大脑中的老年斑也与这些探针结合。不过，这种结合并不只表示cadherin mRNA，因为ISH同时检测到了反义和有义cRNA探针。我们的数据表明，虽然反义 cRNA 探针能有效检测到 AD 脑细胞中的粘连蛋白 mRNA 表达，但它们与老年斑的结合可能并不专门表示粘连蛋白 mRNA 的表达。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.