Evaluation of Autophagy in Conjunctival Fibroblasts.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-03-19 DOI:10.1007/7651_2024_523
Parvaneh Mehrbod, Paola Brun, Umberto Rosani, Andrea Leonardi, Saeid Ghavami
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Abstract

Vernal keratoconjunctivitis (VKC) is a serious eye allergy characterized by poorly understood pathogenic mechanisms and a lack of effective treatments. Autophagy, a process involved in both triggering and suppressing immune and inflammatory responses, plays a role in VKC's pathophysiology. Understanding autophagy's involvement in VKC could lead to new treatment possibilities, such as utilizing specific topical substances to induce or inhibit autophagy and prevent severe complications of this eye condition. In our current protocol, we present a robust methodology established in our laboratory for studying autophagy in primary conjunctival fibroblasts. We assess autophagy through techniques like immunocytochemistry, immunoblotting, and qPCR.

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评估结膜成纤维细胞的自噬作用
春季角膜结膜炎(VKC)是一种严重的眼部过敏症,其发病机制尚不清楚,也缺乏有效的治疗方法。自噬是一种参与触发和抑制免疫和炎症反应的过程,在 VKC 的病理生理学中扮演着重要角色。了解自噬在 VKC 中的作用可为治疗提供新的可能性,例如利用特定的外用物质来诱导或抑制自噬,从而预防这种眼病的严重并发症。在目前的研究方案中,我们介绍了本实验室为研究原代结膜成纤维细胞自噬而建立的一套可靠方法。我们通过免疫细胞化学、免疫印迹和 qPCR 等技术来评估自噬。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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