Determination of Maximum Tolerable Cold Ischemia Time in a Mouse Model of Cervical Heterotopic Uterus Transplantation.

IF 5.3 2区医学 Q1 IMMUNOLOGY Transplantation Pub Date : 2024-09-01 Epub Date: 2024-03-19 DOI:10.1097/TP.0000000000004979

Xin Luo, Shengnan Yu, Bing Liu, Qisheng Zheng, Xin Zhou, Ke An, Jiaying Zhong, Licheng Wu, Helong Dai, Zhongquan Qi, Junjie Xia

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Abstract

Background: Uterus transplantation (UTx) is an emerging treatment for uterine factor infertility. Determining the maximum tolerable cold ischemia time is crucial for successful UTx. However, the limit for cold ischemia in the uterus is unclear. This study aimed to examine cold ischemia's effects on mouse uteri and identify the maximum cold ischemia duration that uteri can endure.

Methods: We systematically assessed the tolerance of mouse uteri to extended cold ischemia, 24 h, 36 h, and 48 h, using the cervical heterotopic UTx model. Multiple indicators were used to evaluate ischemia-reperfusion injury, including reperfusion duration, macroscopic examination, oxidative stress, inflammation, and histopathology. The function of transplants was evaluated through estrous cycle monitoring and embryo transfer.

Results: Mouse uteri subjected to 48 h of cold ischemia exhibited significant delays and insufficiencies in reperfusion, substantial tissue necrosis, and loss of the estrous cycle. Conversely, uteri that underwent cold ischemia within 36 h showed long survival, regular estrous cycles, and fertility.

Conclusions: Our study demonstrated that mouse uteri can endure at least 36 h of cold ischemia, extending the known limits for cold ischemia and providing a pivotal reference for research on the prevention and treatment of cold ischemic injury in UTx.

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确定宫颈异位子宫移植小鼠模型的最长耐受冷缺血时间

背景：子宫移植（UTx）是治疗子宫因素性不孕的一种新兴疗法。确定可耐受的最长冷缺血时间是成功进行子宫移植的关键。然而，子宫冷缺血的极限尚不明确。本研究旨在研究冷缺血对小鼠子宫的影响，并确定子宫可承受的最长冷缺血时间：方法：我们使用宫颈异位UTx模型系统地评估了小鼠子宫对24小时、36小时和48小时长时间冷缺血的耐受性。评估缺血再灌注损伤的指标包括再灌注持续时间、宏观检查、氧化应激、炎症和组织病理学。通过监测发情周期和胚胎移植来评估移植的功能：结果：经过 48 小时冷缺血的小鼠子宫表现出明显的再灌注延迟和不足、大量组织坏死和发情周期丧失。相反，在 36 小时内接受冷缺血的子宫存活时间长，发情周期正常，并具有生育能力：我们的研究表明，小鼠子宫至少可以承受36小时的冷缺血，这扩大了已知的冷缺血极限，为UTx冷缺血损伤的预防和治疗研究提供了重要参考。

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来源期刊

Transplantation 医学-免疫学

CiteScore

8.50

自引率

11.30%

发文量

1906

审稿时长

1 months

期刊介绍： The official journal of The Transplantation Society, and the International Liver Transplantation Society, Transplantation is published monthly and is the most cited and influential journal in the field, with more than 25,000 citations per year. Transplantation has been the trusted source for extensive and timely coverage of the most important advances in transplantation for over 50 years. The Editors and Editorial Board are an international group of research and clinical leaders that includes many pioneers of the field, representing a diverse range of areas of expertise. This capable editorial team provides thoughtful and thorough peer review, and delivers rapid, careful and insightful editorial evaluation of all manuscripts submitted to the journal. Transplantation is committed to rapid review and publication. The journal remains competitive with a time to first decision of fewer than 21 days. Transplantation was the first in the field to offer CME credit to its peer reviewers for reviews completed. The journal publishes original research articles in original clinical science and original basic science. Short reports bring attention to research at the forefront of the field. Other areas covered include cell therapy and islet transplantation, immunobiology and genomics, and xenotransplantation.