(-)-epicatechin treatment did not modify the thermogenic pathway in the gastrocnemius muscle of male rat offspring obeses by programming.

IF 1.8 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Journal of Developmental Origins of Health and Disease Pub Date : 2024-03-19 DOI:10.1017/S2040174424000072
María Elena Tejeda, Sergio De Los Santos, Ramón Mauricio Coral-Vázquez, Ana Álvarez-Chávez, Carlos Palma Flores, Elena Zambrano, Juan Pablo Méndez, Patricia Canto
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Abstract

The aim of this study was to analyse the expression of genes related to the regulation of energy metabolism in skeletal muscle tissue by comparing male offspring in two age groups [at 110 and 245 postnatal days (pnd)] from a mother with obesity induced by a high-fat diet and (-)-epicatechin (Epi) administration. Four groups of six male offspring from different litters were randomly selected for the control groups [C and offspring of mothers with maternal obesity (MO)] or Epi intervention groups. We evaluated the effect of Epi on gastrocnemius tissue by analysing the mRNA and protein expression levels of Fndc5/irisin, Pgc-1α, Ucp3, and Sln. Epi significantly increased the Pgc-1α protein in the MO group of offspring at 110 pnd (p < 0.036, MO vs. MO+Epi), while at 245 pnd, Epi increased Fndc5/irisin mRNA expression in the MO+Epi group versus the MO group (p = 0.006).No differences were detected in Fndc5/irisin, Ucp3 or Sln mRNA or protein levels (including Pgc-1α mRNA) in the offspring at 110 pnd or in Pgc-1α, Ucp3, or Sln mRNA or protein levels (including Fndc5/irisin protein) at 245 pnd among the experimental groups. In conclusion, (-)-epicatechin treatment increased Fndc5/irisin mRNA expression and Pgc-α protein levels in the gastrocnemius muscle of offspring at postnatal days 110 and 245. Furthermore, it is suggested that the flavonoid effect in a model of obesity and its impact on thermogenesis in skeletal muscle are regulated by a different pathway than Fndc5/irisin.

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通过编程,(-)-表儿茶素处理不会改变肥胖雄性后代大鼠腓肠肌的产热途径。
本研究旨在通过比较两个年龄组(出生后 110 天和 245 天 (pnd))的雄性后代,分析与骨骼肌组织能量代谢调控有关的基因的表达。从不同窝中随机抽取四组六只雄性后代,分别作为对照组[C和母亲肥胖(MO)的后代]或Epi干预组。我们通过分析Fndc5/鸢尾素、Pgc-1α、Ucp3和Sln的mRNA和蛋白表达水平,评估了Epi对腓肠肌组织的影响。在110 pnd时,Epi明显增加了MO组后代的Pgc-1α蛋白(P < 0.036,MO组与MO+Epi组相比),而在245 pnd时,Epi增加了MO+Epi组与MO组相比的Fndc5/irisin mRNA表达(P = 0.006)。110 pnd时,子代的Fndc5/irisin、Ucp3或Sln mRNA或蛋白质水平(包括Pgc-1α mRNA),以及245 pnd时,各实验组的Pgc-1α、Ucp3或Sln mRNA或蛋白质水平(包括Fndc5/irisin蛋白质)均未发现差异。总之,(-)-表儿茶素能增加出生后第110天和第245天后代腓肠肌中Fndc5/鸢尾素mRNA的表达和Pgc-α蛋白水平。此外,研究还表明,类黄酮在肥胖模型中的作用及其对骨骼肌产热的影响是由与Fndc5/鸢尾素不同的途径调节的。
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来源期刊
Journal of Developmental Origins of Health and Disease
Journal of Developmental Origins of Health and Disease PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
3.80
自引率
0.00%
发文量
145
审稿时长
6-12 weeks
期刊介绍: JDOHaD publishes leading research in the field of Developmental Origins of Health and Disease (DOHaD). The Journal focuses on the environment during early pre-natal and post-natal animal and human development, interactions between environmental and genetic factors, including environmental toxicants, and their influence on health and disease risk throughout the lifespan. JDOHaD publishes work on developmental programming, fetal and neonatal biology and physiology, early life nutrition, especially during the first 1,000 days of life, human ecology and evolution and Gene-Environment Interactions. JDOHaD also accepts manuscripts that address the social determinants or education of health and disease risk as they relate to the early life period, as well as the economic and health care costs of a poor start to life. Accordingly, JDOHaD is multi-disciplinary, with contributions from basic scientists working in the fields of physiology, biochemistry and nutrition, endocrinology and metabolism, developmental biology, molecular biology/ epigenetics, human biology/ anthropology, and evolutionary developmental biology. Moreover clinicians, nutritionists, epidemiologists, social scientists, economists, public health specialists and policy makers are very welcome to submit manuscripts. The journal includes original research articles, short communications and reviews, and has regular themed issues, with guest editors; it is also a platform for conference/workshop reports, and for opinion, comment and interaction.
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