Molecular mapping and candidate gene identification of two major quantitative trait loci associated with silique length in oilseed rape (Brassica napus L.)

IF 2.6 3区农林科学 Q1 AGRONOMY Molecular Breeding Pub Date : 2024-03-19 DOI:10.1007/s11032-024-01464-x

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Abstract

Rapeseed is a significant global source of plant oil. Silique size, particularly silique length (SL), impacts rapeseed yield. SL is a typical quantitative trait controlled by multiple genes. In our previous study, we constructed a DH population of 178 families known as the 158A-SGDH population. In this study, through SL QTL mapping, we identified twenty-six QTL for SL across five replicates in two environments. A QTL meta-analysis revealed eight consensus QTL, including two major QTL: cqSL.A02-1 (11.32–16.44% of PVE for SL), and cqSL.C06-1 (10.90–11.95% of PVE for SL). Based on biparental resequencing data and microcollinearity analysis of target regions in Brassica napus and Arabidopsis, we identified 11 candidate genes at cqSL.A02-1 and 6 candidate genes at cqSL.C06-1, which are potentially associated with silique development. Furthermore, transcriptome analysis of silique valves from both parents on the 14th, 21st, and 28th days after pollination (DAP) combined with gene function annotation revealed three significantly differentially expressed genes at cqSL.A02-1, BnaA02G0058500ZS, BnaA02G0060100ZS, and BnaA02G0060900ZS. Only the gene BnaC06G0283800ZS showed significant differences in parental transcription at cqSL.C06-1. Two tightly linked insertion-deletion markers for the cqSL.A02-1 and cqSL.C06-1 loci were developed. Using these two QTL, we generated four combinations: A02^SGDH284C06^158A, A02^SGDH284C06^SGDH284, A02^158AC06^158A, and A02^158AC06^SGDH284. Subsequent analysis identified an ideal QTL combination, A02^158AC06^SGDH284, which exhibited the longest SL of this type, reaching 6.06 ± 0.10 cm, significantly surpassing the other three combinations. The results will provide the basis for the cloning of SL-related genes of rapeseed, along with the development of functional markers of target genes and the breeding of rapeseed varieties.

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与油菜（Brassica napus L.）子房长度相关的两个主要数量性状位点的分子图谱和候选基因鉴定

摘要油菜籽是全球植物油的重要来源。颖果大小，尤其是颖果长度（SL）对油菜籽产量有影响。SL是由多个基因控制的典型数量性状。在之前的研究中，我们构建了一个由 178 个家系组成的 DH 群体，称为 158A-SGDH 群体。在本研究中，通过SL QTL作图，我们在两个环境的五个重复中发现了26个SL QTL。通过 QTL 元分析，我们发现了八个共识 QTL，其中包括两个主要 QTL：cqSL.A02-1（占 SL PVE 的 11.32-16.44%）和 cqSL.C06-1（占 SL PVE 的 10.90-11.95%）。根据双亲重测序数据以及对甘蓝型油菜和拟南芥目标区域的微ollinearity 分析，我们在 cqSL.A02-1 和 cqSL.C06-1 分别发现了 11 个候选基因和 6 个候选基因，这些基因可能与韧皮部发育有关。此外，结合基因功能注释对授粉后第 14、21 和 28 天的双亲颖果瓣进行转录组分析发现，cqSL.A02-1 有三个显著差异表达的基因：BnaA02G0058500ZS、BnaA02G0060100ZS 和 BnaA02G0060900ZS。只有基因 BnaC06G0283800ZS 在 cqSL.C06-1 中的亲本转录有显著差异。我们为 cqSL.A02-1 和 cqSL.C06-1 基因座开发了两个紧密相连的插入-缺失标记。利用这两个 QTL，我们产生了四个组合：A02SGDH284C06158A、A02SGDH284C06SGDH284、A02158AC06158A 和 A02158AC06SGDH284。随后的分析确定了一个理想的 QTL 组合 A02158AC06SGDH284，它表现出该类型中最长的 SL，达到 6.06 ± 0.10 厘米，大大超过了其他三个组合。这些结果将为油菜籽SL相关基因的克隆、目标基因功能标记的开发和油菜籽品种的培育提供依据。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.