The Cytotoxic Activity of Phenazine Compounds from Pseudomonas chlororaphis subsp. aurantiaca against the HeLa Cell Line

Abstract

In this investigation, the cytotoxic activity of phenazine compounds from different bacterial strains of Pseudomonas chlororaphis subsp. aurantiaca against the HeLa cervical adenocarcinoma cell line was studied. The cytotoxic concentrations of phenazines against HeLa cells were 300 μg/mL. After incubation with phenazines, cytological preparations of HeLa cells showing the presence of apoptotic bodies were obtained. The effect of phenazines on HeLa cells led to a change in the expression of their ABC transporter genes (abcc1 and abcg2) and tp53. The activity of tp53 increased almost 13-fold, while the expression of the abcc1 and abcg2 genes decreased. The activation of tp53 is one of the probable causes of apoptotic death of HeLa cells in the presence of phenazine compounds from the bacterium P. chlororaphis subsp. aurantiaca.