A. F. Vanin, A. A. Abramov, A. B. Vagapov, A. A. Timoshin, A. V. Pekshev, V. L. Lakomkin, E. K. Ruuge
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引用次数: 0
Abstract
The reason for the lack of the hypotensive effect of gaseous NO, as introduced by inhalation into the bodies of animals and humans, has been identified. Since this defect was completely eliminated by inhalation of NO simultaneous with intravenous administration of low molecular mass thiol solutions to animals (rats), it is concluded that gaseous NO entering through the lungs into the blood circulating in a large circle of blood circulation converts into nitrosonium cation (NO+) as a result of single-electron oxidation, which is unable to exert vasodilating and thereby hypotensive effects on animals and humans. The binding of NO+ to low molecular mass thiols leads to its transformation into S-nitrosothiols, followed by the release of this nitrosyl agent in the form of neutral NO molecules characterized by hypotensive activity. The formation of dinitrosyl iron complexes with thiol-containing ligands in the blood and tissues of organs of experimental animals in these experiments, which could cause a hypotensive effect, was not detected. The hypotensive effect of inhaled NO, which was found in the lungs, could be due to the penetration of NO through the outer wall of blood vessels with subsequent activation of the enzyme guanylate cyclase, an inducer of vasodilation and hypotension, directly in the walls of blood vessels.
摘要 通过吸入动物和人体内的气态氮氧化物缺乏降压作用的原因已经查明。由于在给动物(大鼠)静脉注射低分子质量硫醇溶液的同时吸入 NO 完全消除了这一缺陷,因此得出结论:气态 NO 经肺进入血液循环的大循环圈后,由于单电子氧化作用而转化为亚硝基锍阳离子(NO+),这种阳离子无法对动物和人类产生血管扩张作用,因而也就无法产生降血压作用。NO+ 与低分子质量硫醇结合后会转化为 S-亚硝基硫醇,然后以具有降压活性的中性 NO 分子的形式释放出这种亚硝基物质。在这些实验中,没有检测到在实验动物的血液和器官组织中与含硫醇配体形成二亚硝基铁络合物,而这种络合物可能会导致降血压效应。在肺部发现的吸入 NO 的降压作用可能是由于 NO 穿透血管外壁,随后直接在血管壁上激活了鸟苷酸环化酶(血管舒张和降压的诱导剂)。
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍:
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.