A Decrease in the Background Production of Reactive Oxygen Species by Neutrophils after the Action of Hypomagnetic Field Is Not Accompanied by a Violation of Their Chemiluminescent Response to Respiratory Burst Activators

Abstract

It has been shown that a decrease in background production of reactive oxygen species in peritoneal neutrophils of mice after a short-term (40 min) stay in hypomagnetic conditions (residual field of ~10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, was not accompanied by a violation of the chemiluminescent response to respiratory burst activators of formylated peptide N-formyl–Met–Leu–Phe (fMLP) and forbol ether forbol-12-meristate-13-acetate (FMA). These results were obtained by activated chemiluminescence method using lucigenin and luminol and various combinations of activators for the production of reactive oxygen species (forbol-12-meristate-13-acetate and/or N-formyl–Met–Leu–Phe). The study, together with the previously obtained results, makes it possible to exclude the systems controlling the respiratory burst in neutrophils from the main targets and acceptors that react to short-term deprivation of a magnetic field.