B M'rabet Samaali, A Loulou, A MougouHamdane, S Kallel
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引用次数: 0
Abstract
Grapevine fanleaf virus (GFLV) is one of the most severe virus diseases of grapevines, causing fanleaf degeneration that is transmitted by Xiphinema index. This paper aims to isolate Xiphinema species from Tunisian vineyard soil samples and assess their ability to acquire and transmit GFLV under natural and controlled conditions. Based on morphological and morphometric analyses, Tunisian dagger nematodes were identified as X. index and Xiphinema italiae. These results were confirmed with molecular identification tools using species-specific polymerase chain reaction primers. The total RNA of GFLV was extracted from specimens of Xiphinema and amplified based on real-time polymerase chain reaction using virus-specific primers. Our results showed that X. index could acquire and transmit the viral particles of GFLV. This nepovirus was not detected in X. italiae, under natural conditions; however, under controlled conditions, this nematode was able to successfully acquire and transmit the viral particles of GFLV.
期刊介绍：
Journal of Helminthology publishes original papers and review articles on all aspects of pure and applied helminthology, particularly those helminth parasites of environmental health, medical or veterinary importance. Research papers on helminths in wildlife hosts, including plant and insect parasites, are also published along with taxonomic papers contributing to the systematics of a group. The journal will be of interest to academics and researchers involved in the fields of human and veterinary parasitology, public health, microbiology, ecology and biochemistry.
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