Significant heightened methylation levels of RUNX3 gene promoter in patients with systemic lupus erythematosus.

IF 1.9 4区 医学 Q3 RHEUMATOLOGY Lupus Pub Date : 2024-05-01 Epub Date: 2024-03-21 DOI:10.1177/09612033241241850
Naeim Ehtesham, Samira Alesaeidi, Dorita Mohammad Zadeh, Mozhdeh Saghaei, Maryam Fakhri, Zahra Bayati, Emran Esmaeilzadeh, Meysam Mosallaei
{"title":"Significant heightened methylation levels of <i>RUNX3</i> gene promoter in patients with systemic lupus erythematosus.","authors":"Naeim Ehtesham, Samira Alesaeidi, Dorita Mohammad Zadeh, Mozhdeh Saghaei, Maryam Fakhri, Zahra Bayati, Emran Esmaeilzadeh, Meysam Mosallaei","doi":"10.1177/09612033241241850","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Researchers are actively investigating new diagnostic and prognostic biomarkers that offer improved sensitivity and specificity for systemic lupus erythematosus (SLE). One area of interest is DNA methylation changes. Previous studies have shown a connection between the <i>RUNX3</i> gene dysfunction and SLE. In this study, the focus was on examining the methylation level of the <i>RUNX3</i> promoter in peripheral blood mononuclear cells (PBMCs) of SLE patients and healthy individuals.</p><p><strong>Methods: </strong>A total of 80 individuals diagnosed with SLE from Iran, along with 77 healthy individuals, were included. The methylation levels of the <i>RUNX3</i> gene in the extracted DNA were evaluated using the MethyQESD method. To determine the diagnostic effectiveness of the <i>RUNX3</i> promoter methylation level, a receiver operating characteristic (ROC) curve was generated.</p><p><strong>Results: </strong>The methylation of the RUNX3 promoter was found to be significantly higher in patients with SLE compared to healthy individuals (<i>p</i> < .001). This difference in methylation levels was observed between SLE patients and healthy individuals and between SLE patients with renal involvement and those without renal involvement (86.29 ± 10.30 vs 40.28 ± 24.21, <i>p</i> < .001). ROC analyses revealed that the methylation level of the RUNX3 promoter had a diagnostic power of 0.769 [95% CI (0.681-0.814)] for SLE. Additionally, there was a positive correlation between the RUNX3 methylation level and levels of creatinine and C4.</p><p><strong>Conclusion: </strong>The findings of this study emphasize the potential use of <i>RUNX3</i> methylation levels in PBMCs of SLE patients as biomarkers for diagnosing the disease, predicting renal damage, and assessing disease activity.</p>","PeriodicalId":18044,"journal":{"name":"Lupus","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lupus","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/09612033241241850","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"RHEUMATOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: Researchers are actively investigating new diagnostic and prognostic biomarkers that offer improved sensitivity and specificity for systemic lupus erythematosus (SLE). One area of interest is DNA methylation changes. Previous studies have shown a connection between the RUNX3 gene dysfunction and SLE. In this study, the focus was on examining the methylation level of the RUNX3 promoter in peripheral blood mononuclear cells (PBMCs) of SLE patients and healthy individuals.

Methods: A total of 80 individuals diagnosed with SLE from Iran, along with 77 healthy individuals, were included. The methylation levels of the RUNX3 gene in the extracted DNA were evaluated using the MethyQESD method. To determine the diagnostic effectiveness of the RUNX3 promoter methylation level, a receiver operating characteristic (ROC) curve was generated.

Results: The methylation of the RUNX3 promoter was found to be significantly higher in patients with SLE compared to healthy individuals (p < .001). This difference in methylation levels was observed between SLE patients and healthy individuals and between SLE patients with renal involvement and those without renal involvement (86.29 ± 10.30 vs 40.28 ± 24.21, p < .001). ROC analyses revealed that the methylation level of the RUNX3 promoter had a diagnostic power of 0.769 [95% CI (0.681-0.814)] for SLE. Additionally, there was a positive correlation between the RUNX3 methylation level and levels of creatinine and C4.

Conclusion: The findings of this study emphasize the potential use of RUNX3 methylation levels in PBMCs of SLE patients as biomarkers for diagnosing the disease, predicting renal damage, and assessing disease activity.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
系统性红斑狼疮患者的 RUNX3 基因启动子甲基化水平显著增高。
目的:研究人员正在积极研究新的诊断和预后生物标志物,以提高系统性红斑狼疮(SLE)的敏感性和特异性。DNA甲基化变化是其中一个值得关注的领域。先前的研究表明,RUNX3 基因功能紊乱与系统性红斑狼疮之间存在联系。本研究的重点是检测系统性红斑狼疮患者和健康人外周血单核细胞(PBMC)中 RUNX3 启动子的甲基化水平:方法:共纳入 80 名来自伊朗的系统性红斑狼疮确诊患者和 77 名健康人。采用 MethyQESD 方法评估了提取的 DNA 中 RUNX3 基因的甲基化水平。为确定 RUNX3 启动子甲基化水平的诊断效果,生成了接收者操作特征曲线(ROC):结果:与健康人相比,系统性红斑狼疮患者的 RUNX3 启动子甲基化水平明显更高(p < .001)。在系统性红斑狼疮患者和健康人之间,以及有肾脏受累的系统性红斑狼疮患者和没有肾脏受累的系统性红斑狼疮患者之间,都观察到了这种甲基化水平的差异(86.29 ± 10.30 vs 40.28 ± 24.21,p < .001)。ROC 分析显示,RUNX3 启动子甲基化水平对系统性红斑狼疮的诊断率为 0.769 [95% CI (0.681-0.814)]。此外,RUNX3甲基化水平与肌酐和C4水平呈正相关:本研究的结果强调了系统性红斑狼疮患者血浆细胞中 RUNX3 甲基化水平作为诊断疾病、预测肾损伤和评估疾病活动性的生物标志物的潜在用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Lupus
Lupus 医学-风湿病学
CiteScore
4.20
自引率
11.50%
发文量
225
审稿时长
1 months
期刊介绍: The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…
期刊最新文献
Health seeking behaviour and diagnostic delays in SLE: A multi-ethnic Malaysian cohort study. Rituximab in lupus anticoagulant hypoprothrombinemia syndrome: A case report. Different patterns of longitudinal changes in antinuclear antibodies titers in children with systemic lupus erythematosus and Sjögren's syndrome. Optical coherence tomography angiography findings of systemic lupus erythematosus patients and the effect of neuropsychiatric involvement on it. Correlation between ABO blood group and clinical characteristics of patients with lupus nephritis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1