Simplified Quantification of Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the Nematode Caenorhabditis elegans.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-03-21 DOI:10.1007/7651_2024_536
Sarah R Fausett, Caroline A Laury, Rachel E Magallon, Christian Braendle
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Abstract

Germ stem cell (GSC) niches are fundamental for the maintenance of the immortal germ cell lineage across generations. In the nematode Caenorhabditis elegans, the simple GSC system has served as an important model for understanding stem cell biology and underlying genetic architecture. GSC niche activity in C. elegans is highly sensitive to subtle environmental and genetic variation. Quantifying variation in the C. elegans GSC niche is therefore essential; however, most methods to do so remain labor-intensive and time-consuming when screening large numbers of individuals. Here, we present a simple and efficient method to estimate the size of the C. elegans GSC niche progenitor pool. This method is ideal for detecting differences in progenitor pool size among different genotypes and environmental treatments during medium- to high-throughput applications such as forward genetic screens and quantitative genetics.

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线虫 Caenorhabditis elegans 中作为生殖干细胞生态位活动指标的祖细胞区大小的简化定量。
生殖干细胞(GSC)龛位是跨代维持不朽生殖细胞系的基础。在线虫秀丽隐杆线虫(Caenorhabditis elegans)中,简单的GSC系统是了解干细胞生物学和基本遗传结构的重要模型。elegans 的 GSC niche 活动对微妙的环境和遗传变异高度敏感。因此,对 elegans GSC 生态位中的变异进行定量至关重要;然而,在筛选大量个体时,大多数方法仍然耗费大量人力和时间。在此,我们提出了一种简单高效的方法来估算秀丽隐杆线虫 GSC 龛祖细胞池的大小。这种方法非常适合在前向遗传筛选和定量遗传学等中高通量应用中检测不同基因型和环境处理之间祖细胞池大小的差异。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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