Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Research Communications Pub Date : 2024-01-01 DOI:10.22099/MBRC.2024.48583.1882
Hamid Behrouj, Mehran Erfani, Pooneh Mokarram
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Abstract

Cholesterol and the Wnt/β-catenin pathway have an effective role in the proliferation, survival, drug resistance, immune exhaustion, and metastasis of all types of cancer cells. Considering the role of LDLR and LRP6 proteins in cholesterol uptake by cells and activation of Wnt/β-catenin pathway, this study aims to examine the gene expression of LDLR and LRP6 in cell lines of breast cancer. Human breast cancer cell lines MCF7, MD468 and SKBR3 were cultured in suitable conditions and after extracting total RNA from them, real-Time PCR was used to measure the levels of gene expression for LDLR and LRP6. Our results showed that the expression of LDLR and LRP6 genes is significantly increased in MCF7 and MD468 cells compared to SKBR3 cells. These results suggest that LRP6 and LDLR can be considered as a therapeutic target in tumors that have a genetic profile similar to MCF7 and MD468 cells.

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研究乳腺癌细胞系中低密度脂蛋白受体(LDLR)和低密度脂蛋白受体相关蛋白 6(LRP6)基因的表达。
胆固醇和Wnt/β-catenin通路在各类癌细胞的增殖、存活、耐药、免疫衰竭和转移中发挥着重要作用。考虑到 LDLR 和 LRP6 蛋白在细胞吸收胆固醇和激活 Wnt/β-catenin 通路中的作用,本研究旨在检测 LDLR 和 LRP6 在乳腺癌细胞系中的基因表达。在合适的条件下培养人乳腺癌细胞株 MCF7、MD468 和 SKBR3,提取它们的总 RNA 后,使用 real-Time PCR 检测 LDLR 和 LRP6 的基因表达水平。结果表明,与 SKBR3 细胞相比,MCF7 和 MD468 细胞中 LDLR 和 LRP6 基因的表达量明显增加。这些结果表明,LRP6 和 LDLR 可被视为与 MCF7 和 MD468 细胞遗传特征相似的肿瘤的治疗靶点。
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来源期刊
Molecular Biology Research Communications
Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
0.00%
发文量
12
期刊介绍: “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.
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