XinJiaCongRongTuSiZiWan protects triptolide-induced rats from oxidative stress injury via mitophagy mediated PINK1/Parkin signaling pathway.

Acta cirurgica brasileira Pub Date : 2024-03-15 eCollection Date: 2024-01-01 DOI:10.1590/acb391424

Yan Jin, Deng Di-Si, Wu Ke-Ming

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Abstract

Purpose: XinJiaCongRongTuSiZiWan (XJCRTSZW) is a traditional Chinese medicine compound for invigorating the kidney, nourishing blood, and promoting blood circulation. This study aimed to explore the effect of XJCRTSZW on triptolide (TP)-induced oxidative stress injury.

Methods: Adult female Sprague-Dawley rats and human ovarian granulosa cell lines were treated with TP and XJCRTSZW. Hematoxylin and eosin staining, enzyme-linked immunosorbent assay, flow cytometry, CCK-8, JC-1 staining, transmission electron microscopy, reverse transcription-quantitative polymerase chain reaction, and Western blotting were performed in this study.

Results: XJCRTSZW treatment observably ameliorated the TP-induced pathological symptoms. Furthermore, XJCRTSZW treatment observably enhanced the TP-induced reduction of estradiol, anti-Mullerian hormone, progesterone, superoxide dismutase, ATP content, mitochondrial membrane potential, p62, and Hsp60 mRNA, and protein levels in vivo and in vitro (p < 0.05). However, TP-induced elevation of follicle stimulating hormone and luteinizing hormone concentrations, malondialdehyde levels, reactive oxygen species levels, apoptosis rate, mitophagy, and the mRNA and protein expressions of LC3-II/LC3-I, PTEN-induced kinase 1 (PINK1), and Parkin were decreased (p < 0.05). In addition, XJCRTSZW treatment markedly increased cell viability in vitro (p < 0.05).

Conclusions: XJCRTSZW protects TP-induced rats from oxidative stress injury via the mitophagy-mediated PINK1/Parkin pathway.

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辛甲聪荣土司丸通过有丝分裂介导的PINK1/Parkin信号通路保护三苯氧胺诱导的大鼠免受氧化应激损伤。

目的：辛栀补血汤是一种具有补肾、养血、活血作用的中药复方制剂。本研究旨在探讨 XJCRTSZW 对三苯氧胺（TP）诱导的氧化应激损伤的影响：方法：将成年雌性 Sprague-Dawley 大鼠和人卵巢颗粒细胞系分别用 TP 和 XJCRTSZW 处理。结果：XJCRTSZW 处理可观察到卵巢颗粒细胞的氧化应激损伤：结果：XJCRTSZW能明显改善TP诱导的病理症状。此外，XJCRTSZW 治疗明显提高了 TP 诱导的体内和体外雌二醇、抗穆勒氏激素、孕酮、超氧化物歧化酶、ATP 含量、线粒体膜电位、p62 和 Hsp60 mRNA 和蛋白水平的降低（p < 0.05）。然而，TP 诱导的促卵泡激素和促黄体生成素浓度、丙二醛水平、活性氧水平、细胞凋亡率、有丝分裂以及 LC3-II/LC3-I、PTEN 诱导的激酶 1 (PINK1) 和 Parkin 的 mRNA 和蛋白表达均下降（p < 0.05）。此外，XJCRTSZW还能显著提高体外细胞活力（p < 0.05）：XJCRTSZW通过有丝分裂介导的PINK1/Parkin通路保护TP诱导的大鼠免受氧化应激损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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