Expression of AQP-10, -11 and -12 in the rat stria vascularis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-01 Epub Date: 2024-03-21 DOI:10.1080/00016489.2024.2329286

Rie Ichikawa, Taizo Takeda, Akinobu Kakigi, Hiroaki Ito, Taisuke Kobayashi, Masamitsu Hyodo

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Abstract

Background: Water homeostasis is essential for inner ear function. Several aquaporins (AQPs), which are water transport proteins in the cell or plasma membrane, have been reported in the lateral wall of the rat inner ear (cochlea). However, the presence of AQP-10, -11 and -12 has not been reported in the rat stria vascularis (SV) to date.

Aims/objectives: We have aimed to clarify the expression of AQP-10, -11 and -12 in the cochlea lateral wall.

Materials and methods: Using Wistar rats, we examined the expression of AQP-10, -11 and -12 in the cochlea lateral wall using molecular approaches and immunohistochemistry.

Results: AQP-11 was molecular biologically expressed, but the expression of AQP-10 and -12 was not observed. Immunohistochemically, AQP-11 was diffusely localized in the basal cells and marginal cells of the rat SV but was not expressed at the apical site of marginal cells with double staining. The expression of AQP-10 and -12 was not observed.

Conclusions and significance: Only AQP-11 was expressed in the basal cells and marginal cells, but it was not expressed at the apical site of marginal cells. Based on this study, AQP-11 may not have an important role in water flux between the perilymph and endolymph.

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大鼠血管纹中 AQP-10、-11 和 -12 的表达。

背景：水的平衡对内耳功能至关重要。据报道，大鼠内耳（耳蜗）侧壁存在多种水汽蛋白（AQPs），它们是细胞膜或质膜上的水转运蛋白。然而，迄今为止尚未有关于大鼠血管纹（SV）中存在 AQP-10、-11 和 -12 的报道：材料与方法：材料和方法：我们使用Wistar大鼠，采用分子方法和免疫组化技术检测了AQP-10、-11和-12在耳蜗侧壁的表达：结果：AQP-11有分子生物学表达，但未观察到AQP-10和-12的表达。免疫组化结果显示，AQP-11弥漫定位于大鼠SV的基底细胞和边缘细胞，但在边缘细胞的顶端部位没有双染表达。未观察到 AQP-10 和 -12 的表达：结论与意义：只有 AQP-11 在基底细胞和边缘细胞中表达，但在边缘细胞的顶端部位没有表达。根据这项研究，AQP-11 在周淋巴和内淋巴之间的水通量中可能不起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.